PCR-Probe Capture Hybridization Assay and Statistical Model for SEN Virus Prevalence Estimation

Yasuhito Tanaka; Ruth Pfeiffer; Anthony E.T. Yeo; Masashi Mizokami; Brian R. Edlin; Philip Castle; Thomas R. O'Brien; Harvey J. Alter; J. Wai Kuo Shih

doi:10.1002/jmv.20067

PCR-Probe Capture Hybridization Assay and Statistical Model for SEN Virus Prevalence Estimation

Yasuhito Tanaka, Ruth Pfeiffer, Anthony E.T. Yeo, Masashi Mizokami, Brian R. Edlin, Philip Castle, Thomas R. O'Brien, Harvey J. Alter, J. Wai Kuo Shih

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

SEN viruses (SENV) are newly discovered blood-borne single-stranded circular DNA viruses that may play a role in liver disease. To date, no serologic assays are available for the detection of SENV antigens or antibodies. We report on a rapid and sensitive molecular assay for the detection of four SENV strains (SENV-A, -C, -D, -H). This method uses PCR with universal primers and microwell capture hybridization with type-specific probes. Cut-off points to define "infected" based on chemiluminescence readings were determined from a statistical mixture model applied to samples from 300 injection drug users (IDUs) in San Francisco. Based on the estimated cut-off points, we examined the prevalence of SENV infection among 232 healthy US blood donors and assessed sensitivity and specificity of the assay in a small validation sample of infected individuals with partial sequence information.

Original language	English (US)
Pages (from-to)	123-130
Number of pages	8
Journal	Journal of Medical Virology
Volume	73
Issue number	1
DOIs	https://doi.org/10.1002/jmv.20067
State	Published - May 2004
Externally published	Yes

Keywords

Cut-off value
Injection drug users
Mixture model
SENV
Sensitivity

ASJC Scopus subject areas

Virology
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1002/jmv.20067

Cite this

@article{fb20e98a4d6942b7a77afc32a275f3cb,

title = "PCR-Probe Capture Hybridization Assay and Statistical Model for SEN Virus Prevalence Estimation",

abstract = "SEN viruses (SENV) are newly discovered blood-borne single-stranded circular DNA viruses that may play a role in liver disease. To date, no serologic assays are available for the detection of SENV antigens or antibodies. We report on a rapid and sensitive molecular assay for the detection of four SENV strains (SENV-A, -C, -D, -H). This method uses PCR with universal primers and microwell capture hybridization with type-specific probes. Cut-off points to define {"}infected{"} based on chemiluminescence readings were determined from a statistical mixture model applied to samples from 300 injection drug users (IDUs) in San Francisco. Based on the estimated cut-off points, we examined the prevalence of SENV infection among 232 healthy US blood donors and assessed sensitivity and specificity of the assay in a small validation sample of infected individuals with partial sequence information.",

keywords = "Cut-off value, Injection drug users, Mixture model, SENV, Sensitivity",

author = "Yasuhito Tanaka and Ruth Pfeiffer and Yeo, {Anthony E.T.} and Masashi Mizokami and Edlin, {Brian R.} and Philip Castle and O'Brien, {Thomas R.} and Alter, {Harvey J.} and Shih, {J. Wai Kuo}",

year = "2004",

month = may,

doi = "10.1002/jmv.20067",

language = "English (US)",

volume = "73",

pages = "123--130",

journal = "Journal of Medical Virology",

issn = "0146-6615",

publisher = "Wiley-Liss Inc.",

number = "1",

}

TY - JOUR

T1 - PCR-Probe Capture Hybridization Assay and Statistical Model for SEN Virus Prevalence Estimation

AU - Tanaka, Yasuhito

AU - Pfeiffer, Ruth

AU - Yeo, Anthony E.T.

AU - Mizokami, Masashi

AU - Edlin, Brian R.

AU - Castle, Philip

AU - O'Brien, Thomas R.

AU - Alter, Harvey J.

AU - Shih, J. Wai Kuo

PY - 2004/5

Y1 - 2004/5

N2 - SEN viruses (SENV) are newly discovered blood-borne single-stranded circular DNA viruses that may play a role in liver disease. To date, no serologic assays are available for the detection of SENV antigens or antibodies. We report on a rapid and sensitive molecular assay for the detection of four SENV strains (SENV-A, -C, -D, -H). This method uses PCR with universal primers and microwell capture hybridization with type-specific probes. Cut-off points to define "infected" based on chemiluminescence readings were determined from a statistical mixture model applied to samples from 300 injection drug users (IDUs) in San Francisco. Based on the estimated cut-off points, we examined the prevalence of SENV infection among 232 healthy US blood donors and assessed sensitivity and specificity of the assay in a small validation sample of infected individuals with partial sequence information.

AB - SEN viruses (SENV) are newly discovered blood-borne single-stranded circular DNA viruses that may play a role in liver disease. To date, no serologic assays are available for the detection of SENV antigens or antibodies. We report on a rapid and sensitive molecular assay for the detection of four SENV strains (SENV-A, -C, -D, -H). This method uses PCR with universal primers and microwell capture hybridization with type-specific probes. Cut-off points to define "infected" based on chemiluminescence readings were determined from a statistical mixture model applied to samples from 300 injection drug users (IDUs) in San Francisco. Based on the estimated cut-off points, we examined the prevalence of SENV infection among 232 healthy US blood donors and assessed sensitivity and specificity of the assay in a small validation sample of infected individuals with partial sequence information.

KW - Cut-off value

KW - Injection drug users

KW - Mixture model

KW - SENV

KW - Sensitivity

UR - http://www.scopus.com/inward/record.url?scp=1842455891&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1842455891&partnerID=8YFLogxK

U2 - 10.1002/jmv.20067

DO - 10.1002/jmv.20067

M3 - Article

C2 - 15042659

AN - SCOPUS:1842455891

SN - 0146-6615

VL - 73

SP - 123

EP - 130

JO - Journal of Medical Virology

JF - Journal of Medical Virology

IS - 1

ER -

PCR-Probe Capture Hybridization Assay and Statistical Model for SEN Virus Prevalence Estimation

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this