Partial excision of bla KPC from Tn4401 in carbapenem-resistant Klebsiella pneumoniae

Liang Chen; Kalyan D. Chavda; José R. Mediavilla; Michael R. Jacobs; Michael H. Levi; Robert A. Bonomo; Barry N. Kreiswirth

doi:10.1128/AAC.06182-11

Partial excision of bla _KPC from Tn4401 in carbapenem-resistant Klebsiella pneumoniae

Liang Chen, Kalyan D. Chavda, José R. Mediavilla, Michael R. Jacobs, Michael H. Levi, Robert A. Bonomo, Barry N. Kreiswirth

Pathology

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

We describe a novel Tn4401 variant (Tn4401d) in epidemic Klebsiella pneumoniae clone ST258, from which a partial bla _KPCfragment has been excised along with ISKpn7 and a partial tnpA fragment. Nested-PCR experiments confirmed that this region can be removed from distinct Tn4401 isoforms in both K. pneumoniae and Escherichia coli. This study highlights that the region surrounding bla _KPC is undergoing recombination and that Tn4401 itself is heterogeneous and highly plastic.

Original language	English (US)
Pages (from-to)	1635-1638
Number of pages	4
Journal	Antimicrobial agents and chemotherapy
Volume	56
Issue number	3
DOIs	https://doi.org/10.1128/AAC.06182-11
State	Published - Mar 2012

ASJC Scopus subject areas

Pharmacology
Pharmacology (medical)
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1128/AAC.06182-11

Cite this

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abstract = "We describe a novel Tn4401 variant (Tn4401d) in epidemic Klebsiella pneumoniae clone ST258, from which a partial bla KPCfragment has been excised along with ISKpn7 and a partial tnpA fragment. Nested-PCR experiments confirmed that this region can be removed from distinct Tn4401 isoforms in both K. pneumoniae and Escherichia coli. This study highlights that the region surrounding bla KPC is undergoing recombination and that Tn4401 itself is heterogeneous and highly plastic.",

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AU - Mediavilla, José R.

AU - Jacobs, Michael R.

AU - Levi, Michael H.

AU - Bonomo, Robert A.

AU - Kreiswirth, Barry N.

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AB - We describe a novel Tn4401 variant (Tn4401d) in epidemic Klebsiella pneumoniae clone ST258, from which a partial bla KPCfragment has been excised along with ISKpn7 and a partial tnpA fragment. Nested-PCR experiments confirmed that this region can be removed from distinct Tn4401 isoforms in both K. pneumoniae and Escherichia coli. This study highlights that the region surrounding bla KPC is undergoing recombination and that Tn4401 itself is heterogeneous and highly plastic.

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