TY - JOUR
T1 - Origin of endothelial progenitors in human postnatal bone marrow
AU - Reyes, Morayma
AU - Dudek, Arkadiusz
AU - Jahagirdar, Balkrishna
AU - Koodie, Lisa
AU - Marker, Paul H.
AU - Verfaillie, Catherine M.
PY - 2002
Y1 - 2002
N2 - This study demonstrates that a CD34-, vascular endothelial cadherin- (VE-cadherin-), AC133+, and fetal liver kinase+ (Flk1+) multipotent adult progenitor cell (MAPC) that copurifies with mesenchymal stem cells from postnatal human bone marrow (BM) is a progenitor for angioblasts. In vitro, MAPCs cultured with VEGF differentiate into CD34+, VE-cadherin+, Flk1+ cells - a phenotype that would be expected for angioblasts. They subsequently differentiate into cells that express endothelial markers, function in vitro as mature endothelial cells, and contribute to neoangiogenesis in vivo during tumor angiogenesis and wound healing. This in vitro model of preangioblast-to-endothelium differentiation should prove very useful in studying commitment to the angioblast and beyond. In vivo, MAPCs can differentiate in response to local cues into endothelial cells that contribute to neoangiogenesis in tumors. Because MAPCs can be expanded in culture without obvious senescence for more than 80 population doublings, they may be an important source of endothelial cells for cellular pro- or anti-angiogenic therapies.
AB - This study demonstrates that a CD34-, vascular endothelial cadherin- (VE-cadherin-), AC133+, and fetal liver kinase+ (Flk1+) multipotent adult progenitor cell (MAPC) that copurifies with mesenchymal stem cells from postnatal human bone marrow (BM) is a progenitor for angioblasts. In vitro, MAPCs cultured with VEGF differentiate into CD34+, VE-cadherin+, Flk1+ cells - a phenotype that would be expected for angioblasts. They subsequently differentiate into cells that express endothelial markers, function in vitro as mature endothelial cells, and contribute to neoangiogenesis in vivo during tumor angiogenesis and wound healing. This in vitro model of preangioblast-to-endothelium differentiation should prove very useful in studying commitment to the angioblast and beyond. In vivo, MAPCs can differentiate in response to local cues into endothelial cells that contribute to neoangiogenesis in tumors. Because MAPCs can be expanded in culture without obvious senescence for more than 80 population doublings, they may be an important source of endothelial cells for cellular pro- or anti-angiogenic therapies.
UR - http://www.scopus.com/inward/record.url?scp=0036171009&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036171009&partnerID=8YFLogxK
U2 - 10.1172/JCI0214327
DO - 10.1172/JCI0214327
M3 - Article
C2 - 11827993
AN - SCOPUS:0036171009
SN - 0021-9738
VL - 109
SP - 337
EP - 346
JO - Journal of Clinical Investigation
JF - Journal of Clinical Investigation
IS - 3
ER -