On the mechanism of production of tandem genetic duplications in phage lambda

Scott W. Emmons, Virginia MacCosham, Robert L. Baldwin

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


We have asked whether the mechanism by which tandem genetic duplications arise in the chromosome of phage lambda is inter- or intramolecular. Two parental phages carrying genetic markers at opposite ends of the phage chromosome have been grown in mixed infection, and progeny phages carrying newly-arising tandem duplications have been analysed to determine whether they carry the markers in parental or recombinant configuration. Ordinary genetic recombination of the markers has been prevented by mutations in the phage and host. Phages carrying tandem duplications are isolated by use of CsCl density gradients and an Escherichia coli strain that does not plate deletion phages. Of the duplication mutants isolated under these conditions, 13% carry the input markers in recombinant configuration. This suggests that tandem duplications can be produced via an intermolecular route which joins sequences originally present on different DNA molecules.

Original languageEnglish (US)
Pages (from-to)83-89
Number of pages7
JournalJournal of Molecular Biology
Issue number1
StatePublished - Jun 15 1975
Externally publishedYes

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology


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