Nucleolar localization of early tRNA processing

Edouard Bertrand, Felicia Houser-Scott, Ann Kendall, Robert H. Singer, David R. Engelke

Research output: Contribution to journalArticlepeer-review

182 Scopus citations


There is little information as to the location of early tRNA biosynthesis. Using fluorescent in situ hybridization in the budding yeast, Saccharomyces cerevisiae, examples of nuclear pre-tRNAs are shown to reside primarily in the nucleoli. We also probed the RNA subunit of RNase P. The majority of the signal from RNase P probes was nucleolar, with less intense signals in the nucleoplasm. These results demonstrate that a major portion of the tRNA processing pathway is compartmentalized in nucleoli with rRNA synthesis and ribosomal assembly. The spatial juxtaposition suggests the possibility of direct coordination between tRNA and ribosome biosynthesis.

Original languageEnglish (US)
Pages (from-to)2463-2468
Number of pages6
JournalGenes and Development
Issue number16
StatePublished - Aug 15 1998


  • Nucleolus
  • RNase P
  • tRNA processing

ASJC Scopus subject areas

  • General Medicine


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