Abstract
A simple procedure for sample preparation of human plasma by two stages of ultra-filtration using one device is described. Our approach is useful for nondigest liberation of biomarkers bound to albumin and other plasma proteins. The analyte contained in the ultrafiltrate can be directly analyzed without additional sample preparation, and quantified by 2-D RP-RP LC/MS.
Original language | English (US) |
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Pages (from-to) | 245-248 |
Number of pages | 4 |
Journal | Journal of Separation Science |
Volume | 30 |
Issue number | 2 |
DOIs | |
State | Published - Feb 2007 |
Keywords
- 2-D chromatography
- Albumin
- Biomarkers
- LC/MS
- Ultrafiltration
ASJC Scopus subject areas
- Analytical Chemistry
- Filtration and Separation