Non-Invasive Near-Infrared Optogenetics: Aspirational Dream or Approaching Feasibility

Diana Galiakhmetova; Viktor Dremin; Aleksandr Koviarov; Dmitrii Stoliarov; Neville Ngum; Rheinallt Parri; Andrei Gorodetsky; Marios Maimaris; Daria M. Shcherbakova; Mikhail Baloban; Vladislav V. Verkhusha; Sergei Sokolovski; Edik Rafailov

doi:10.1117/12.3016681

Non-Invasive Near-Infrared Optogenetics: Aspirational Dream or Approaching Feasibility

Diana Galiakhmetova, Viktor Dremin, Aleksandr Koviarov, Dmitrii Stoliarov, Neville Ngum, Rheinallt Parri, Andrei Gorodetsky, Marios Maimaris, Daria M. Shcherbakova, Mikhail Baloban, Vladislav V. Verkhusha, Sergei Sokolovski, Edik Rafailov

Genetics

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

This research focuses on the development of a non-invasive/minimally invasive optogenetic technique. The study delves into how visible (VIS) and near-infrared (NIR) light interacts with ex vivo mouse head tissues, highlighting the advantages of the NIR-II biological window for deeper tissue penetration and reduced light absorption and scattering. Our computer simulations and experimental results demonstrated that over 12% of initial light irradiation passes through 1 mm tissue (skin and skull), reaching the brain cortex, potentially enabling minimally invasive neural activation. Moreover, this work reveals the nonlinear optical properties of genetically engineered truncated monomeric and dimeric bacterial phytochromes, demonstrating their photoconversion efficiency of up to 73% in the NIR-II range and potential for optogenetics. This discovery opens new avenues in advanced neurostimulation and biomedical research by enhancing tissue penetration and minimizing invasiveness.

Original language	English (US)
Title of host publication	Neurophotonics II
Editors	Tomas Cizmar, Tommaso Fellin
Publisher	SPIE
ISBN (Electronic)	9781510673328
DOIs	https://doi.org/10.1117/12.3016681
State	Published - 2024
Event	Neurophotonics II 2024 - Strasbourg, France Duration: Apr 8 2024 → Apr 9 2024

Publication series

Name	Proceedings of SPIE - The International Society for Optical Engineering
Volume	13007
ISSN (Print)	0277-786X
ISSN (Electronic)	1996-756X

Conference

Conference	Neurophotonics II 2024
Country/Territory	France
City	Strasbourg
Period	4/8/24 → 4/9/24

Keywords

light-tissue interaction
monomeric phytochrome
optogenetics
penetration depth
second near-infrared window
two-photon absorption
two-photon fluorescence
ultrashort pulsed fiber laser

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Computer Science Applications
Applied Mathematics
Electrical and Electronic Engineering

Access to Document

10.1117/12.3016681

Cite this

Galiakhmetova, D., Dremin, V., Koviarov, A., Stoliarov, D., Ngum, N., Parri, R., Gorodetsky, A., Maimaris, M., Shcherbakova, D. M., Baloban, M., Verkhusha, V. V., Sokolovski, S., & Rafailov, E. (2024). Non-Invasive Near-Infrared Optogenetics: Aspirational Dream or Approaching Feasibility. In T. Cizmar, & T. Fellin (Eds.), Neurophotonics II Article 1300703 (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 13007). SPIE. https://doi.org/10.1117/12.3016681

Non-Invasive Near-Infrared Optogenetics: Aspirational Dream or Approaching Feasibility. / Galiakhmetova, Diana; Dremin, Viktor; Koviarov, Aleksandr et al.
Neurophotonics II. ed. / Tomas Cizmar; Tommaso Fellin. SPIE, 2024. 1300703 (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 13007).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Galiakhmetova, D, Dremin, V, Koviarov, A, Stoliarov, D, Ngum, N, Parri, R, Gorodetsky, A, Maimaris, M, Shcherbakova, DM , Baloban, M , Verkhusha, VV, Sokolovski, S & Rafailov, E 2024, Non-Invasive Near-Infrared Optogenetics: Aspirational Dream or Approaching Feasibility. in T Cizmar & T Fellin (eds), Neurophotonics II., 1300703, Proceedings of SPIE - The International Society for Optical Engineering, vol. 13007, SPIE, Neurophotonics II 2024, Strasbourg, France, 4/8/24. https://doi.org/10.1117/12.3016681

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abstract = "This research focuses on the development of a non-invasive/minimally invasive optogenetic technique. The study delves into how visible (VIS) and near-infrared (NIR) light interacts with ex vivo mouse head tissues, highlighting the advantages of the NIR-II biological window for deeper tissue penetration and reduced light absorption and scattering. Our computer simulations and experimental results demonstrated that over 12% of initial light irradiation passes through 1 mm tissue (skin and skull), reaching the brain cortex, potentially enabling minimally invasive neural activation. Moreover, this work reveals the nonlinear optical properties of genetically engineered truncated monomeric and dimeric bacterial phytochromes, demonstrating their photoconversion efficiency of up to 73% in the NIR-II range and potential for optogenetics. This discovery opens new avenues in advanced neurostimulation and biomedical research by enhancing tissue penetration and minimizing invasiveness.",

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AU - Ngum, Neville

AU - Parri, Rheinallt

AU - Gorodetsky, Andrei

AU - Maimaris, Marios

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AU - Sokolovski, Sergei

AU - Rafailov, Edik

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Non-Invasive Near-Infrared Optogenetics: Aspirational Dream or Approaching Feasibility

Abstract

Publication series

Conference

Keywords

ASJC Scopus subject areas

Access to Document

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