TY - JOUR
T1 - Near-infrared fluorescent proteins engineered from bacterial phytochromes
AU - Shcherbakova, Daria M.
AU - Baloban, Mikhail
AU - Verkhusha, Vladislav V.
N1 - Funding Information:
We thank Yoshihiro Miwa (University of Tsukuba, Japan), Eva M. Sevick-Muraca (University of Texas, TX), Yaoliang Tang (Georgia Regents University, GA) and Assou El-Battari (Aix-Marseille University, France) for sharing their images of the iRFP713-expressing mouse models. This work was supported by the grants GM073913, GM108579 and CA164468 from the US National Institutes of Health and ERC-2013-ADG-340233 from the EU FP7 program.
Publisher Copyright:
© 2015 Elsevier Ltd.
PY - 2015/8/1
Y1 - 2015/8/1
N2 - Near-infrared fluorescent proteins (NIR FPs), photoactivatable NIR FPs and NIR reporters of protein-protein interactions developed from bacterial phytochrome photoreceptors (BphPs) have advanced non-invasive deep-tissue imaging. Here we provide a brief guide to the BphP-derived NIR probes with an emphasis on their in vivo applications. We describe phenotypes of NIR FPs and their photochemical and intracellular properties. We discuss NIR FP applications for imaging of various cell types, tissues and animal models in basic and translational research. In this discussion, we focus on NIR FPs that efficiently incorporate endogenous biliverdin chromophore and therefore can be used as straightforward as GFP-like proteins. We also overview a usage of NIR FPs in different imaging platforms, from planar epifluorescence to tomographic and photoacoustic technologies.
AB - Near-infrared fluorescent proteins (NIR FPs), photoactivatable NIR FPs and NIR reporters of protein-protein interactions developed from bacterial phytochrome photoreceptors (BphPs) have advanced non-invasive deep-tissue imaging. Here we provide a brief guide to the BphP-derived NIR probes with an emphasis on their in vivo applications. We describe phenotypes of NIR FPs and their photochemical and intracellular properties. We discuss NIR FP applications for imaging of various cell types, tissues and animal models in basic and translational research. In this discussion, we focus on NIR FPs that efficiently incorporate endogenous biliverdin chromophore and therefore can be used as straightforward as GFP-like proteins. We also overview a usage of NIR FPs in different imaging platforms, from planar epifluorescence to tomographic and photoacoustic technologies.
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U2 - 10.1016/j.cbpa.2015.06.005
DO - 10.1016/j.cbpa.2015.06.005
M3 - Review article
C2 - 26115447
AN - SCOPUS:84935005822
SN - 1367-5931
VL - 27
SP - 52
EP - 63
JO - Current Opinion in Chemical Biology
JF - Current Opinion in Chemical Biology
ER -