N-terminal protein characterization by mass spectrometry after cyanogen bromide cleavage using combined microscale liquid- and solid-phase derivatization

Keyphrases

• Mass Spectrometry 100%
• Cyanogen Bromide Cleavage 100%
• Terminal Protein 100%
• Specimen Preparation 100%
• Liquid Phase 100%
• Solid-phase Analytical Derivatization 100%
• Protein Characterization 100%
• Cyanogen Bromide 66%
• N-terminal Peptide 66%
• Thiols 33%
• Hydroxylamine 33%
• Ethyl 33%
• In Situ 33%
• N-terminus 33%
• Disulfide 33%
• Hydroxyl Group 33%
• Amines 33%
• Acetylation 33%
• C-terminal Peptide 33%
• Model Proteins 33%
• Thiol Groups 33%
• Acylation 33%
• Derivatization 33%
• Sepharose 33%
• Pipette Tip 33%
• Reversed-phase Supports 33%
• N-terminal Fragment 33%
• Protein Digest 33%
• Matrix-assisted Laser Desorption Ionization (MALDI) 33%
• Flow-through 33%
• Covalent Chromatography 33%
• Peptide Isolation 33%
• Solid-phase Reaction 33%
• Reaction Scheme 33%
• One-pot Reaction 33%
• Mass Spectra 33%
• Peptide Mixtures 33%
• Reductive Release 33%
• Biological Laboratory 33%

Chemistry

• Mass Spectrometry 100%
• Derivatization 100%
• Protein Characterization 100%
• Peptide 100%
• Sample Preparation Method 60%
• Matrix-Assisted Laser Desorption-Ionization 20%
• Disulfide 20%
• Ethyl 20%
• Hydroxyl Group 20%
• Thiol Group 20%
• Hydroxylamine 20%
• Solid State Reaction 20%
• One Pot Reaction 20%
• Thiol 20%
• Chromatography 20%
• Amine 20%
• Acetylation 20%
• Acylation 20%
• Reaction Scheme 20%