Mycobacterium smegmatis biofilm formation and sliding motility are affected by the serine/threonine protein kinase PknF

Radha Gopalaswamy; Sujatha Narayanan; William R. Jacobs; Yossef Av-Gay

doi:10.1111/j.1574-6968.2007.00989.x

Mycobacterium smegmatis biofilm formation and sliding motility are affected by the serine/threonine protein kinase PknF

Radha Gopalaswamy
, Sujatha Narayanan
, William R. Jacobs
, Yossef Av-Gay

Microbiology & Immunology

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

Eighteen 'eukaryotic-like' serine/threonine kinases are present in the Mycobacterium smegmatis genome. One of them encoded by the ORF 3677 demonstrates high similarity to the Mycobacterium tuberculosis protein kinase PknF. A merodiploid strain was generated, which showed reduced growth associated with irregular cell structure. The merodiploid strain displayed altered colony morphology, defective sliding motility and biofilm formation. These data indicate a role for PknF in biofilm formation, possibly associated with alterations in glycopeptidolipid composition.

Original language	English (US)
Pages (from-to)	121-127
Number of pages	7
Journal	FEMS Microbiology Letters
Volume	278
Issue number	1
DOIs	https://doi.org/10.1111/j.1574-6968.2007.00989.x
State	Published - Jan 2008

Keywords

Biofilm
Cell morphology
GPLs
Mycobacteria
Serine/threonine kinase
Sliding motility

ASJC Scopus subject areas

General Medicine

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1111/j.1574-6968.2007.00989.x

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abstract = "Eighteen 'eukaryotic-like' serine/threonine kinases are present in the Mycobacterium smegmatis genome. One of them encoded by the ORF 3677 demonstrates high similarity to the Mycobacterium tuberculosis protein kinase PknF. A merodiploid strain was generated, which showed reduced growth associated with irregular cell structure. The merodiploid strain displayed altered colony morphology, defective sliding motility and biofilm formation. These data indicate a role for PknF in biofilm formation, possibly associated with alterations in glycopeptidolipid composition.",

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AU - Narayanan, Sujatha

AU - Jacobs, William R.

AU - Av-Gay, Yossef

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AB - Eighteen 'eukaryotic-like' serine/threonine kinases are present in the Mycobacterium smegmatis genome. One of them encoded by the ORF 3677 demonstrates high similarity to the Mycobacterium tuberculosis protein kinase PknF. A merodiploid strain was generated, which showed reduced growth associated with irregular cell structure. The merodiploid strain displayed altered colony morphology, defective sliding motility and biofilm formation. These data indicate a role for PknF in biofilm formation, possibly associated with alterations in glycopeptidolipid composition.

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KW - Cell morphology

KW - GPLs

KW - Mycobacteria

KW - Serine/threonine kinase

KW - Sliding motility

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Mycobacterium smegmatis biofilm formation and sliding motility are affected by the serine/threonine protein kinase PknF

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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