Multiscale photoacoustic tomography of a genetically encoded near-infrared FRET biosensor

Research output: Chapter in Book/Report/Conference proceedingConference contribution


Photoacoustic tomography with genetically encoded near-infrared probes enables visualization of specific cell populations in vivo at high resolution in deep tissues. Here, we report a near-infrared Forster resonance energy transfer biosensor based on a pair of the near-infrared fluorescent proteins, miRFP670-iRFP720, which enables dynamic photoacoustic imaging of active biological processes. Specifically, we detected apoptosis in single cells at a resolution of 3m in a mouse ear and mouse brain tumors (>3 mm in depth) at a spatial resolution of 150m. These results open the way for high-resolution photoacoustic imaging of dynamic biological processes in deep tissues.

Original languageEnglish (US)
Title of host publicationPhotons Plus Ultrasound
Subtitle of host publicationImaging and Sensing 2022
EditorsAlexander A. Oraevsky, Lihong V. Wang
ISBN (Electronic)9781510647916
StatePublished - 2022
EventPhotons Plus Ultrasound: Imaging and Sensing 2022 - Virtual, Online
Duration: Feb 20 2022Feb 24 2022

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


ConferencePhotons Plus Ultrasound: Imaging and Sensing 2022
CityVirtual, Online


  • FRET
  • PAT
  • biosensor
  • photoacoustic microscopy

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging


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