Multiplex real-time PCR for detection of an epidemic KPC-producing Klebsiella pneumoniae ST258 clone

Liang Chen; Kalyan D. Chavda; José R. Mediavilla; Yanan Zhao; Henry S. Fraimow; Stephen G. Jenkins; Michael H. Levi; Tao Hong; Albert D. Rojtman; Christine C. Ginocchio; Robert A. Bonomo; Barry N. Kreiswirth

doi:10.1128/AAC.00316-12

Multiplex real-time PCR for detection of an epidemic KPC-producing Klebsiella pneumoniae ST258 clone

Liang Chen
, Kalyan D. Chavda
, José R. Mediavilla
, Yanan Zhao
, Henry S. Fraimow
, Stephen G. Jenkins
, Michael H. Levi
, Tao Hong
, Albert D. Rojtman
, Christine C. Ginocchio
, Robert A. Bonomo
, Barry N. Kreiswirth

Pathology

Research output: Contribution to journal › Article › peer-review

48 Scopus citations

Abstract

We describe a multiplex real-time PCR assay capable of identifying both the epidemic Klebsiella pneumoniae ST258 clone and bla_KPC carbapenemase genes in a single reaction. The assay displayed excellent sensitivity (100%) and specificity (100%) for identification of ST258 clone and bla_KPC in a collection of 75 K. pneumoniae isolates comprising 41 sequence types. Our results suggest that this assay is an effective tool for surveillance of this clone among carbapenem-resistant K. pneumoniae clinical isolates.

Original language	English (US)
Pages (from-to)	3444-3447
Number of pages	4
Journal	Antimicrobial agents and chemotherapy
Volume	56
Issue number	6
DOIs	https://doi.org/10.1128/AAC.00316-12
State	Published - Jun 2012

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

ASJC Scopus subject areas

Pharmacology
Pharmacology (medical)
Infectious Diseases

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10.1128/AAC.00316-12

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Chen, L., Chavda, K. D., Mediavilla, J. R., Zhao, Y., Fraimow, H. S., Jenkins, S. G., Levi, M. H., Hong, T., Rojtman, A. D., Ginocchio, C. C., Bonomo, R. A., & Kreiswirth, B. N. (2012). Multiplex real-time PCR for detection of an epidemic KPC-producing Klebsiella pneumoniae ST258 clone. Antimicrobial agents and chemotherapy, 56(6), 3444-3447. https://doi.org/10.1128/AAC.00316-12

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title = "Multiplex real-time PCR for detection of an epidemic KPC-producing Klebsiella pneumoniae ST258 clone",

abstract = "We describe a multiplex real-time PCR assay capable of identifying both the epidemic Klebsiella pneumoniae ST258 clone and blaKPC carbapenemase genes in a single reaction. The assay displayed excellent sensitivity (100\%) and specificity (100\%) for identification of ST258 clone and blaKPC in a collection of 75 K. pneumoniae isolates comprising 41 sequence types. Our results suggest that this assay is an effective tool for surveillance of this clone among carbapenem-resistant K. pneumoniae clinical isolates.",

author = "Liang Chen and Chavda, \{Kalyan D.\} and Mediavilla, \{Jos{\'e} R.\} and Yanan Zhao and Fraimow, \{Henry S.\} and Jenkins, \{Stephen G.\} and Levi, \{Michael H.\} and Tao Hong and Rojtman, \{Albert D.\} and Ginocchio, \{Christine C.\} and Bonomo, \{Robert A.\} and Kreiswirth, \{Barry N.\}",

year = "2012",

month = jun,

doi = "10.1128/AAC.00316-12",

language = "English (US)",

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pages = "3444--3447",

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AU - Chavda, Kalyan D.

AU - Mediavilla, José R.

AU - Zhao, Yanan

AU - Fraimow, Henry S.

AU - Jenkins, Stephen G.

AU - Levi, Michael H.

AU - Hong, Tao

AU - Rojtman, Albert D.

AU - Ginocchio, Christine C.

AU - Bonomo, Robert A.

AU - Kreiswirth, Barry N.

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AB - We describe a multiplex real-time PCR assay capable of identifying both the epidemic Klebsiella pneumoniae ST258 clone and blaKPC carbapenemase genes in a single reaction. The assay displayed excellent sensitivity (100%) and specificity (100%) for identification of ST258 clone and blaKPC in a collection of 75 K. pneumoniae isolates comprising 41 sequence types. Our results suggest that this assay is an effective tool for surveillance of this clone among carbapenem-resistant K. pneumoniae clinical isolates.

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Multiplex real-time PCR for detection of an epidemic KPC-producing Klebsiella pneumoniae ST258 clone

Abstract

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