TY - JOUR
T1 - Multifunctional biosynthesized silver nanoparticles exhibiting excellent antimicrobial potential against multi-drug resistant microbes along with remarkable anticancerous properties
AU - Jha, Diksha
AU - Thiruveedula, Prasanna Kumar
AU - Pathak, Rajiv
AU - Kumar, Bipul
AU - Gautam, Hemant K.
AU - Agnihotri, Shrish
AU - Sharma, Ashwani Kumar
AU - Kumar, Pradeep
N1 - Publisher Copyright:
© 2017 Elsevier B.V.
PY - 2017/11/1
Y1 - 2017/11/1
N2 - This study demonstrates the therapeutic potential of silver nanoparticles (AgNPs), which were biosynthesized using the extracts of Citrus maxima plant. Characterization through UV–Vis spectrophotometry, Dynamic Light Scattering (DLS), Fourier Transform Infrared spectroscopy (FTIR), X-ray Diffraction (XRD) and Transmission Electron Microscopy (TEM) confirmed the formation of AgNps in nano-size range. These nanoparticles exhibited enhanced antioxidative activity and showed commendable antimicrobial activity against wide range of microbes including multi-drug resistant bacteria that were later confirmed by TEM. These particles exhibited minimal toxicity when cytotoxicity study was performed on normal human lung fibroblast cell line as well as human red blood cells. It was quite noteworthy that these particles showed remarkable cytotoxicity on human fibrosarcoma and mouse melanoma cell line (B16-F10). Additionally, the apoptotic topographies of B16-F10 cells treated with AgNps were confirmed by using acridine orange and ethidium bromide dual dye staining, caspase-3 assay, DNA fragmentation assay followed by cell cycle analysis using fluorescence-activated cell sorting. Taken together, these results advocate promising potential of the biosynthesized AgNps for their use in therapeutic applications.
AB - This study demonstrates the therapeutic potential of silver nanoparticles (AgNPs), which were biosynthesized using the extracts of Citrus maxima plant. Characterization through UV–Vis spectrophotometry, Dynamic Light Scattering (DLS), Fourier Transform Infrared spectroscopy (FTIR), X-ray Diffraction (XRD) and Transmission Electron Microscopy (TEM) confirmed the formation of AgNps in nano-size range. These nanoparticles exhibited enhanced antioxidative activity and showed commendable antimicrobial activity against wide range of microbes including multi-drug resistant bacteria that were later confirmed by TEM. These particles exhibited minimal toxicity when cytotoxicity study was performed on normal human lung fibroblast cell line as well as human red blood cells. It was quite noteworthy that these particles showed remarkable cytotoxicity on human fibrosarcoma and mouse melanoma cell line (B16-F10). Additionally, the apoptotic topographies of B16-F10 cells treated with AgNps were confirmed by using acridine orange and ethidium bromide dual dye staining, caspase-3 assay, DNA fragmentation assay followed by cell cycle analysis using fluorescence-activated cell sorting. Taken together, these results advocate promising potential of the biosynthesized AgNps for their use in therapeutic applications.
KW - Anti-cancerous
KW - Antimicrobial
KW - Antioxidant
KW - Citrus maxima
KW - Multi-drug resistant microorganisms
KW - Silver nanoparticles
UR - http://www.scopus.com/inward/record.url?scp=85024483447&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85024483447&partnerID=8YFLogxK
U2 - 10.1016/j.msec.2017.07.011
DO - 10.1016/j.msec.2017.07.011
M3 - Article
C2 - 28866213
AN - SCOPUS:85024483447
SN - 0928-4931
VL - 80
SP - 659
EP - 669
JO - Materials Science and Engineering C
JF - Materials Science and Engineering C
ER -
