mRNA levels and methylation patterns of the tyrosine aminotransferase gene in aging inbred rats

P. Eline Slagboom; Wiljo J.F. de Leeuw; Jan Vijg

doi:10.1016/0014-5793(90)81136-C

mRNA levels and methylation patterns of the tyrosine aminotransferase gene in aging inbred rats

P. Eline Slagboom
, Wiljo J.F. de Leeuw
, Jan Vijg

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

We have examined the mRNA levels and methylation patterns of the liver-specific tyrosine aminotransferase (TAT) gene in inbred female rats aged 6, 24 and 36 months. Northern hybridization analysis of total RNA showed a 65% decrease in the steady state transcript level of TAT in the liver of 24- and 36-month old rats as compared to 6-month old animals. The TAT gene as studied by Southern hybridization analysis using the isoschizomers Hpa II and Msp I was found to be hypomethylated in the liver as compared to spleen and brain at six CpG sites within the gene. Methylation at these sites remained unchanged during aging.

Original language	English (US)
Pages (from-to)	128-130
Number of pages	3
Journal	FEBS Letters
Volume	269
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(90)81136-C
State	Published - Aug 20 1990
Externally published	Yes

Keywords

Aging
DNA methylation
Tyrosine aminotransferase
mRNA

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(90)81136-C

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title = "mRNA levels and methylation patterns of the tyrosine aminotransferase gene in aging inbred rats",

abstract = "We have examined the mRNA levels and methylation patterns of the liver-specific tyrosine aminotransferase (TAT) gene in inbred female rats aged 6, 24 and 36 months. Northern hybridization analysis of total RNA showed a 65\% decrease in the steady state transcript level of TAT in the liver of 24- and 36-month old rats as compared to 6-month old animals. The TAT gene as studied by Southern hybridization analysis using the isoschizomers Hpa II and Msp I was found to be hypomethylated in the liver as compared to spleen and brain at six CpG sites within the gene. Methylation at these sites remained unchanged during aging.",

keywords = "Aging, DNA methylation, Tyrosine aminotransferase, mRNA",

author = "Slagboom, \{P. Eline\} and \{de Leeuw\}, \{Wiljo J.F.\} and Jan Vijg",

note = "Funding Information: AcknowledgementTsh: is researchw ass upportedb y the Netherlands Organizationf or Advancemenot f Pure Research( NWO) and by the NATO (Grant 86/0668). We thank Dr C. Zurcher for histopathologicaal nalysisa nd helpful discussionsD, r P.L. Pearson and Dr A.G. Uitterlindenf or helpful discussionsD, r W. Schmidf or kindly providing pUTAT0.94, pUTAT2.45 and pUTATl.05, Dr B.D. Young for kindly providing pHR28.1, and Dr J. Kraus for kindly providingp RO21.",

year = "1990",

month = aug,

day = "20",

doi = "10.1016/0014-5793(90)81136-C",

language = "English (US)",

volume = "269",

pages = "128--130",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "John Wiley and Sons Inc.",

number = "1",

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TY - JOUR

T1 - mRNA levels and methylation patterns of the tyrosine aminotransferase gene in aging inbred rats

AU - Slagboom, P. Eline

AU - de Leeuw, Wiljo J.F.

AU - Vijg, Jan

N1 - Funding Information: AcknowledgementTsh: is researchw ass upportedb y the Netherlands Organizationf or Advancemenot f Pure Research( NWO) and by the NATO (Grant 86/0668). We thank Dr C. Zurcher for histopathologicaal nalysisa nd helpful discussionsD, r P.L. Pearson and Dr A.G. Uitterlindenf or helpful discussionsD, r W. Schmidf or kindly providing pUTAT0.94, pUTAT2.45 and pUTATl.05, Dr B.D. Young for kindly providing pHR28.1, and Dr J. Kraus for kindly providingp RO21.

PY - 1990/8/20

Y1 - 1990/8/20

N2 - We have examined the mRNA levels and methylation patterns of the liver-specific tyrosine aminotransferase (TAT) gene in inbred female rats aged 6, 24 and 36 months. Northern hybridization analysis of total RNA showed a 65% decrease in the steady state transcript level of TAT in the liver of 24- and 36-month old rats as compared to 6-month old animals. The TAT gene as studied by Southern hybridization analysis using the isoschizomers Hpa II and Msp I was found to be hypomethylated in the liver as compared to spleen and brain at six CpG sites within the gene. Methylation at these sites remained unchanged during aging.

AB - We have examined the mRNA levels and methylation patterns of the liver-specific tyrosine aminotransferase (TAT) gene in inbred female rats aged 6, 24 and 36 months. Northern hybridization analysis of total RNA showed a 65% decrease in the steady state transcript level of TAT in the liver of 24- and 36-month old rats as compared to 6-month old animals. The TAT gene as studied by Southern hybridization analysis using the isoschizomers Hpa II and Msp I was found to be hypomethylated in the liver as compared to spleen and brain at six CpG sites within the gene. Methylation at these sites remained unchanged during aging.

KW - Aging

KW - DNA methylation

KW - Tyrosine aminotransferase

KW - mRNA

UR - https://www.scopus.com/pages/publications/0025024102

UR - https://www.scopus.com/pages/publications/0025024102#tab=citedBy

U2 - 10.1016/0014-5793(90)81136-C

DO - 10.1016/0014-5793(90)81136-C

M3 - Article

C2 - 1974867

AN - SCOPUS:0025024102

SN - 0014-5793

VL - 269

SP - 128

EP - 130

JO - FEBS Letters

JF - FEBS Letters

IS - 1

ER -

mRNA levels and methylation patterns of the tyrosine aminotransferase gene in aging inbred rats

Abstract

Keywords

ASJC Scopus subject areas

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