Monoclonal antibodies to surface antigens of Mycobacterium tuberculosis and their use in a modified enzyme-linked immunosorbent spot assay for detection of mycobacteria

Aharona Glatman-Freedman; Jean M. Martin; Paul F. Riska; Barry R. Bloom; Arturo Casadevall

doi:10.1128/jcm.34.11.2795-2802.1996

Monoclonal antibodies to surface antigens of Mycobacterium tuberculosis and their use in a modified enzyme-linked immunosorbent spot assay for detection of mycobacteria

Aharona Glatman-Freedman, Jean M. Martin, Paul F. Riska, Barry R. Bloom, Arturo Casadevall

Research output: Contribution to journal › Article › peer-review

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Abstract

Three monoclonal antibodies (MAbs) were generated from splenocytes of a BALB/c mouse immunized with heat-killed Mycobacterium tuberculosis. All three MAbs bound to surface epitopes of M. tuberculosis as shown by whole-cell enzyme-linked immunosorbent assay (ELISA), indirect immunofluorescence, and immunoelectron microscopy. One immunoglobulin M (IgM) MAb bound to lipoarabinomannan, the second IgM MAb bound to mycolyl-arabinogalactan- peptidoglycan complex, and the third MAb, an IgC3, bound to a surface epitope of an uncertain nature. The MAbs demonstrated different cross-reactivity patterns with other mycobacteria. Two of the MAbs were used to develop a modified ELISA spot assay for the detection of mycobacteria.

Original language	English (US)
Pages (from-to)	2795-2802
Number of pages	8
Journal	Journal of Clinical Microbiology
Volume	34
Issue number	11
DOIs	https://doi.org/10.1128/jcm.34.11.2795-2802.1996
State	Published - Nov 1996

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Microbiology (medical)

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10.1128/jcm.34.11.2795-2802.1996

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Monoclonal antibodies to surface antigens of Mycobacterium tuberculosis and their use in a modified enzyme-linked immunosorbent spot assay for detection of mycobacteria. / Glatman-Freedman, Aharona; Martin, Jean M.; Riska, Paul F. et al.
In: Journal of Clinical Microbiology, Vol. 34, No. 11, 11.1996, p. 2795-2802.

Research output: Contribution to journal › Article › peer-review

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Monoclonal antibodies to surface antigens of Mycobacterium tuberculosis and their use in a modified enzyme-linked immunosorbent spot assay for detection of mycobacteria

Abstract

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UN SDGs

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