TY - JOUR
T1 - Monitoring the role of autophagy in C. elegans aging.
AU - Meléndez, Alicia
AU - Hall, David H.
AU - Hansen, Malene
N1 - Funding Information:
The authors wish to thank members of Monica Driscoll’s lab for sharing protocols on measurements of age pigments and life span assays in liquid media. Furthermore, we also thank Hannes Bülow for critical comments on the manuscript. Work in the author's laboratories is supported by grants from the NIH (RR 12596 to D.H.H., 3ROI AG024882‐0451 to A.M.) and the NSF (0818802 to A.M.). M.H. and A.M. are Ellison Medical Foundation New Scholars on Aging.
PY - 2008
Y1 - 2008
N2 - Autophagy plays crucial roles in many biological processes, and recent research points to a possibly conserved role for autophagy in the process of organismal aging. Experiments in the nematode C. elegans suggest that autophagy may be required specifically for longevity pathways that are regulated by environmental signals. Known longevity genes can be assigned to four major longevity pathways/processes: insulin/IGF-1 signaling, dietary restriction, protein translation, and mitochondrial respiration. Of these, reduced insulin/IGF-1 signaling and dietary restriction, but not protein translation inhibition, appear to rely on autophagy to increase life span. Multiple experimental approaches have been used to study autophagy in the context of aging in C. elegans. This chapter describes techniques used to address the link between aging and autophagy in C. elegans. Specifically, we summarize how to examine organismal life span in various longevity mutants and how to visually detect autophagy and auto-lysosomal formation in C. elegans.
AB - Autophagy plays crucial roles in many biological processes, and recent research points to a possibly conserved role for autophagy in the process of organismal aging. Experiments in the nematode C. elegans suggest that autophagy may be required specifically for longevity pathways that are regulated by environmental signals. Known longevity genes can be assigned to four major longevity pathways/processes: insulin/IGF-1 signaling, dietary restriction, protein translation, and mitochondrial respiration. Of these, reduced insulin/IGF-1 signaling and dietary restriction, but not protein translation inhibition, appear to rely on autophagy to increase life span. Multiple experimental approaches have been used to study autophagy in the context of aging in C. elegans. This chapter describes techniques used to address the link between aging and autophagy in C. elegans. Specifically, we summarize how to examine organismal life span in various longevity mutants and how to visually detect autophagy and auto-lysosomal formation in C. elegans.
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M3 - Article
C2 - 19185737
AN - SCOPUS:85057634940
SN - 0076-6879
VL - 451
SP - 493
EP - 520
JO - Methods in enzymology
JF - Methods in enzymology
ER -