Molecular Mechanisms Underlying Pluripotency and Self-Renewal of Embryonic Stem Cells

Fahimeh Varzideh; Jessica Gambardella; Urna Kansakar; Stanislovas S. Jankauskas; Gaetano Santulli

doi:10.3390/ijms24098386

Molecular Mechanisms Underlying Pluripotency and Self-Renewal of Embryonic Stem Cells

Fahimeh Varzideh, Jessica Gambardella, Urna Kansakar, Stanislovas S. Jankauskas, Gaetano Santulli

Medicine

Research output: Contribution to journal › Review article › peer-review

8 Scopus citations

Abstract

Embryonic stem cells (ESCs) are derived from the inner cell mass (ICM) of the blastocyst. ESCs have two distinctive properties: ability to proliferate indefinitely, a feature referred as “self-renewal”, and to differentiate into different cell types, a peculiar characteristic known as “pluripotency”. Self-renewal and pluripotency of ESCs are finely orchestrated by precise external and internal networks including epigenetic modifications, transcription factors, signaling pathways, and histone modifications. In this systematic review, we examine the main molecular mechanisms that sustain self-renewal and pluripotency in both murine and human ESCs. Moreover, we discuss the latest literature on human naïve pluripotency.

Original language	English (US)
Article number	8386
Journal	International Journal of Molecular Sciences
Volume	24
Issue number	9
DOIs	https://doi.org/10.3390/ijms24098386
State	Published - May 2023

Keywords

CRISPR/Cas9
Klf4
Oct4
embryonic stem cell (ESC)
epigenetics
histone modifications
human ESC (hESC) vs. mouse ESC (mESC)
naïve vs. primed pluripotency
pluripotent stem cells (PSC)
self-renewal

ASJC Scopus subject areas

Catalysis
Molecular Biology
Spectroscopy
Computer Science Applications
Physical and Theoretical Chemistry
Organic Chemistry
Inorganic Chemistry

Access to Document

10.3390/ijms24098386

Cite this

@article{a96479ece4f942729a6fcd6f57c842a8,

title = "Molecular Mechanisms Underlying Pluripotency and Self-Renewal of Embryonic Stem Cells",

abstract = "Embryonic stem cells (ESCs) are derived from the inner cell mass (ICM) of the blastocyst. ESCs have two distinctive properties: ability to proliferate indefinitely, a feature referred as “self-renewal”, and to differentiate into different cell types, a peculiar characteristic known as “pluripotency”. Self-renewal and pluripotency of ESCs are finely orchestrated by precise external and internal networks including epigenetic modifications, transcription factors, signaling pathways, and histone modifications. In this systematic review, we examine the main molecular mechanisms that sustain self-renewal and pluripotency in both murine and human ESCs. Moreover, we discuss the latest literature on human na{\"i}ve pluripotency.",

keywords = "CRISPR/Cas9, Klf4, Oct4, embryonic stem cell (ESC), epigenetics, histone modifications, human ESC (hESC) vs. mouse ESC (mESC), na{\"i}ve vs. primed pluripotency, pluripotent stem cells (PSC), self-renewal",

author = "Fahimeh Varzideh and Jessica Gambardella and Urna Kansakar and Jankauskas, {Stanislovas S.} and Gaetano Santulli",

note = "Publisher Copyright: {\textcopyright} 2023 by the authors.",

year = "2023",

month = may,

doi = "10.3390/ijms24098386",

language = "English (US)",

volume = "24",

journal = "International Journal of Molecular Sciences",

issn = "1661-6596",

publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",

number = "9",

}

TY - JOUR

T1 - Molecular Mechanisms Underlying Pluripotency and Self-Renewal of Embryonic Stem Cells

AU - Varzideh, Fahimeh

AU - Gambardella, Jessica

AU - Kansakar, Urna

AU - Jankauskas, Stanislovas S.

AU - Santulli, Gaetano

PY - 2023/5

Y1 - 2023/5

N2 - Embryonic stem cells (ESCs) are derived from the inner cell mass (ICM) of the blastocyst. ESCs have two distinctive properties: ability to proliferate indefinitely, a feature referred as “self-renewal”, and to differentiate into different cell types, a peculiar characteristic known as “pluripotency”. Self-renewal and pluripotency of ESCs are finely orchestrated by precise external and internal networks including epigenetic modifications, transcription factors, signaling pathways, and histone modifications. In this systematic review, we examine the main molecular mechanisms that sustain self-renewal and pluripotency in both murine and human ESCs. Moreover, we discuss the latest literature on human naïve pluripotency.

AB - Embryonic stem cells (ESCs) are derived from the inner cell mass (ICM) of the blastocyst. ESCs have two distinctive properties: ability to proliferate indefinitely, a feature referred as “self-renewal”, and to differentiate into different cell types, a peculiar characteristic known as “pluripotency”. Self-renewal and pluripotency of ESCs are finely orchestrated by precise external and internal networks including epigenetic modifications, transcription factors, signaling pathways, and histone modifications. In this systematic review, we examine the main molecular mechanisms that sustain self-renewal and pluripotency in both murine and human ESCs. Moreover, we discuss the latest literature on human naïve pluripotency.

KW - CRISPR/Cas9

KW - Klf4

KW - Oct4

KW - embryonic stem cell (ESC)

KW - epigenetics

KW - histone modifications

KW - human ESC (hESC) vs. mouse ESC (mESC)

KW - naïve vs. primed pluripotency

KW - pluripotent stem cells (PSC)

KW - self-renewal

UR - http://www.scopus.com/inward/record.url?scp=85159373901&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85159373901&partnerID=8YFLogxK

U2 - 10.3390/ijms24098386

DO - 10.3390/ijms24098386

M3 - Review article

C2 - 37176093

AN - SCOPUS:85159373901

SN - 1661-6596

VL - 24

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

IS - 9

M1 - 8386

ER -

Molecular Mechanisms Underlying Pluripotency and Self-Renewal of Embryonic Stem Cells

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this