Modulation of K+ channel currents by serum amineoxidase in neurons

Lingyun Wu; Mircea Alexandru Mateescu; Xin Tao Wang; Bruno Mondovi; Rui Wang

doi:10.1006/bbrc.1996.0354

Modulation of K⁺ channel currents by serum amineoxidase in neurons

Lingyun Wu
, Mircea Alexandru Mateescu
, Xin Tao Wang
, Bruno Mondovi
, Rui Wang

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

The influence of bovine serum amineoxidase (SAO), a circulating copper-enzyme, on neuronal K⁺ channels is described. Bovine SAO enhanced K⁺ channel currents in N1E-115 neuroblastoma cells in a time-dependent manner. Unlike ceruloplasmin (another copper-protein, shown as depolarizing factor in neurons), SAO had no effect on resting potential of neurons. However, pretreatment of cells with SAO inhibited ceruloplasmin-induced membrane depolarization. Although ceruloplasmin alone inhibited K⁺ channel currents, it further enhanced K⁺ channel currents in the presence of SAO. Therefore, SAO may be another endogenous modulator of neuronal K⁺ channels with effect and mechanisms different from those of ceruloplasmin.

Original language	English (US)
Pages (from-to)	47-52
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	220
Issue number	1
DOIs	https://doi.org/10.1006/bbrc.1996.0354
State	Published - Mar 7 1996
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1006/bbrc.1996.0354

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@article{84c14f75de144f3dbd153ffc95014371,

title = "Modulation of K+ channel currents by serum amineoxidase in neurons",

abstract = "The influence of bovine serum amineoxidase (SAO), a circulating copper-enzyme, on neuronal K+ channels is described. Bovine SAO enhanced K+ channel currents in N1E-115 neuroblastoma cells in a time-dependent manner. Unlike ceruloplasmin (another copper-protein, shown as depolarizing factor in neurons), SAO had no effect on resting potential of neurons. However, pretreatment of cells with SAO inhibited ceruloplasmin-induced membrane depolarization. Although ceruloplasmin alone inhibited K+ channel currents, it further enhanced K+ channel currents in the presence of SAO. Therefore, SAO may be another endogenous modulator of neuronal K+ channels with effect and mechanisms different from those of ceruloplasmin.",

author = "Lingyun Wu and Mateescu, \{Mircea Alexandru\} and Wang, \{Xin Tao\} and Bruno Mondovi and Rui Wang",

note = "Funding Information: This work was supported by a joint MRC (Canada)–CNR (Italy) research agreement (M.A.M and B.M) and by grants from Medical Research Council (MRC), from CNR project on “Biotecnologie e Biologia Moleculare” and from MURST (Italy). R. Wang is a McDonald Scholar of the Heart and Stroke Foundation of Canada.",

year = "1996",

month = mar,

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doi = "10.1006/bbrc.1996.0354",

language = "English (US)",

volume = "220",

pages = "47--52",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Elsevier B.V.",

number = "1",

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TY - JOUR

T1 - Modulation of K+ channel currents by serum amineoxidase in neurons

AU - Wu, Lingyun

AU - Mateescu, Mircea Alexandru

AU - Wang, Xin Tao

AU - Mondovi, Bruno

AU - Wang, Rui

N1 - Funding Information: This work was supported by a joint MRC (Canada)–CNR (Italy) research agreement (M.A.M and B.M) and by grants from Medical Research Council (MRC), from CNR project on “Biotecnologie e Biologia Moleculare” and from MURST (Italy). R. Wang is a McDonald Scholar of the Heart and Stroke Foundation of Canada.

PY - 1996/3/7

Y1 - 1996/3/7

N2 - The influence of bovine serum amineoxidase (SAO), a circulating copper-enzyme, on neuronal K+ channels is described. Bovine SAO enhanced K+ channel currents in N1E-115 neuroblastoma cells in a time-dependent manner. Unlike ceruloplasmin (another copper-protein, shown as depolarizing factor in neurons), SAO had no effect on resting potential of neurons. However, pretreatment of cells with SAO inhibited ceruloplasmin-induced membrane depolarization. Although ceruloplasmin alone inhibited K+ channel currents, it further enhanced K+ channel currents in the presence of SAO. Therefore, SAO may be another endogenous modulator of neuronal K+ channels with effect and mechanisms different from those of ceruloplasmin.

AB - The influence of bovine serum amineoxidase (SAO), a circulating copper-enzyme, on neuronal K+ channels is described. Bovine SAO enhanced K+ channel currents in N1E-115 neuroblastoma cells in a time-dependent manner. Unlike ceruloplasmin (another copper-protein, shown as depolarizing factor in neurons), SAO had no effect on resting potential of neurons. However, pretreatment of cells with SAO inhibited ceruloplasmin-induced membrane depolarization. Although ceruloplasmin alone inhibited K+ channel currents, it further enhanced K+ channel currents in the presence of SAO. Therefore, SAO may be another endogenous modulator of neuronal K+ channels with effect and mechanisms different from those of ceruloplasmin.

UR - https://www.scopus.com/pages/publications/0030009239

UR - https://www.scopus.com/pages/publications/0030009239#tab=citedBy

U2 - 10.1006/bbrc.1996.0354

DO - 10.1006/bbrc.1996.0354

M3 - Article

C2 - 8602855

AN - SCOPUS:0030009239

SN - 0006-291X

VL - 220

SP - 47

EP - 52

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 1

ER -

Modulation of K⁺ channel currents by serum amineoxidase in neurons

Abstract

ASJC Scopus subject areas

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