Modulating cell adhesion and spreading by control of FnIII7-10 orientation on charged self-assembled monolayers (SAMs) of alkanethiolates

Hua Wang, Yi He, Buddy D. Ratner, Shaoyi Jiang

Research output: Contribution to journalArticlepeer-review

39 Scopus citations


In this work, we demonstrate that surface charge can be used to modulate cell adhesion/spreading through the control of the orientation of adsorbed FnIII7-10, which is a cell-adhesive protein containing RGD residues. Carboxylic acid (COOH) and amine (NH2)-terminated self-assembled monolayers (SAMs) of alkanethiolates were used as model negatively and positively charged surfaces, respectively. The adsorbed amount of FnIII 7-10 is controlled to be equivalent on both SAMs as confirmed by the adsorption isotherms determined using I125-radiolabeled FnIII 7-10 The binding of a monoclonal antibody specific for the cell-binding domain of FnIII7-10 was measured by surface plasmon resonance (SPR) to evaluate FnIII7-10 orientations on different SAMs. Results indicate that adsorbed FnIII7-10 on NH2-SAM has an orientation with more cell-binding domains accessible than on COOH-SAM, confirming our predictions from Monte Carlo simulations. Both phase contrast images and Vybrant® MTT cell proliferation assays show that the adhesion/spreading of bovine aortic endothelial cells (BAECs) on the NH 2-SAM is significantly better than that on the COOH-SAM coated with an equivalent amount of FnIII7-10. These results indicate that surface charge can be used to specifically orient cell adhesive proteins such as FnIII7-10, thus providing a promising strategy to increase the activity of materials incorporating biological moieties.

Original languageEnglish (US)
Pages (from-to)672-678
Number of pages7
JournalJournal of Biomedical Materials Research - Part A
Issue number4
StatePublished - Jun 15 2006
Externally publishedYes


  • Biomaterials
  • Cell adhesion and spreading
  • FnIII
  • Protein orientation
  • Self-assembled monolayers

ASJC Scopus subject areas

  • Ceramics and Composites
  • Biomaterials
  • Biomedical Engineering
  • Metals and Alloys


Dive into the research topics of 'Modulating cell adhesion and spreading by control of FnIII7-10 orientation on charged self-assembled monolayers (SAMs) of alkanethiolates'. Together they form a unique fingerprint.

Cite this