TY - JOUR
T1 - Modern fluorescent proteins
T2 - From chromophore formation to novel intracellular applications
AU - Stepanenko, Olesya V.
AU - Stepanenko, Olga V.
AU - Shcherbakova, Daria M.
AU - Kuznetsova, Irina M.
AU - Turoverov, Konstantin K.
AU - Verkhusha, Vladislav V.
PY - 2011/11
Y1 - 2011/11
N2 - The diverse biochemical and photophysical properties of fluorescent proteins (FPs) have enabled the generation of a growing palette of colors, providing unique opportunities for their use in a variety of modern biology applications. Modulation of these FP characteristics is achieved through diversity in both the structure of the chromophore as well as the contacts between the chromophore and the surrounding protein barrel. Here we review our current knowledge of blue, green, and red chromophore formation in permanently emitting FPs, photoactivatable FPs, and fluorescent timers. Progress in understanding the interplay between FP structure and function has allowed the engineering of FPs with many desirable features, and enabled recent advances in microscopy techniques such as super-resolution imaging of single molecules, imaging of protein dynamics, photochromic FRET, deep-tissue imaging, and multicolor two-photon microscopy in live animals.
AB - The diverse biochemical and photophysical properties of fluorescent proteins (FPs) have enabled the generation of a growing palette of colors, providing unique opportunities for their use in a variety of modern biology applications. Modulation of these FP characteristics is achieved through diversity in both the structure of the chromophore as well as the contacts between the chromophore and the surrounding protein barrel. Here we review our current knowledge of blue, green, and red chromophore formation in permanently emitting FPs, photoactivatable FPs, and fluorescent timers. Progress in understanding the interplay between FP structure and function has allowed the engineering of FPs with many desirable features, and enabled recent advances in microscopy techniques such as super-resolution imaging of single molecules, imaging of protein dynamics, photochromic FRET, deep-tissue imaging, and multicolor two-photon microscopy in live animals.
KW - FRET
KW - PALM
KW - PAmcherry
KW - STED
KW - STORM
KW - Two-photon microscopy
KW - mKate
UR - http://www.scopus.com/inward/record.url?scp=80655139792&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80655139792&partnerID=8YFLogxK
U2 - 10.2144/000113765
DO - 10.2144/000113765
M3 - Review article
C2 - 22054544
AN - SCOPUS:80655139792
SN - 0736-6205
VL - 51
SP - 313
EP - 327
JO - BioTechniques
JF - BioTechniques
IS - 5
ER -