miR-4432 Targets FGFBP1 in Human Endothelial Cells

Roberta Avvisato; Pasquale Mone; Stanislovas S. Jankauskas; Fahimeh Varzideh; Urna Kansakar; Jessica Gambardella; Antonio De Luca; Alessandro Matarese; Gaetano Santulli

doi:10.3390/biology12030459

miR-4432 Targets FGFBP1 in Human Endothelial Cells

Roberta Avvisato, Pasquale Mone, Stanislovas S. Jankauskas, Fahimeh Varzideh, Urna Kansakar, Jessica Gambardella, Antonio De Luca, Alessandro Matarese, Gaetano Santulli

Medicine

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

MicroRNAs (miRs) are small non-coding RNAs that modulate the expression of several target genes. Fibroblast growth factor binding protein 1 (FGFBP1) has been associated with endothelial dysfunction at the level of the blood–brain barrier (BBB). However, the underlying mechanisms are mostly unknown and there are no studies investigating the relationship between miRs and FGFBP1. Thus, the overarching aim of the present study was to identify and validate which miR can specifically target FGFBP1 in human brain microvascular endothelial cells, which represent the best in vitro model of the BBB. We were able to identify and validate miR-4432 as a fundamental modulator of FGFBP1 and we demonstrated that miR-4432 significantly reduces mitochondrial oxidative stress, a well-established pathophysiological hallmark of hypertension.

Original language	English (US)
Article number	459
Journal	Biology
Volume	12
Issue number	3
DOIs	https://doi.org/10.3390/biology12030459
State	Published - Mar 2023

Keywords

HUVEC
blood pressure
blood–brain barrier
cerebrovascular disease
endothelial dysfunction
hBMECs
hypertension
miR-4432-3p
miRNA
microRNA

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology
General Immunology and Microbiology
General Agricultural and Biological Sciences

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10.3390/biology12030459

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@article{b4fd79b992ee414ea0d335e208c80a51,

title = "miR-4432 Targets FGFBP1 in Human Endothelial Cells",

abstract = "MicroRNAs (miRs) are small non-coding RNAs that modulate the expression of several target genes. Fibroblast growth factor binding protein 1 (FGFBP1) has been associated with endothelial dysfunction at the level of the blood–brain barrier (BBB). However, the underlying mechanisms are mostly unknown and there are no studies investigating the relationship between miRs and FGFBP1. Thus, the overarching aim of the present study was to identify and validate which miR can specifically target FGFBP1 in human brain microvascular endothelial cells, which represent the best in vitro model of the BBB. We were able to identify and validate miR-4432 as a fundamental modulator of FGFBP1 and we demonstrated that miR-4432 significantly reduces mitochondrial oxidative stress, a well-established pathophysiological hallmark of hypertension.",

keywords = "HUVEC, blood pressure, blood–brain barrier, cerebrovascular disease, endothelial dysfunction, hBMECs, hypertension, miR-4432-3p, miRNA, microRNA",

author = "Roberta Avvisato and Pasquale Mone and Jankauskas, {Stanislovas S.} and Fahimeh Varzideh and Urna Kansakar and Jessica Gambardella and {De Luca}, Antonio and Alessandro Matarese and Gaetano Santulli",

note = "Publisher Copyright: {\textcopyright} 2023 by the authors.",

year = "2023",

month = mar,

doi = "10.3390/biology12030459",

language = "English (US)",

volume = "12",

journal = "Biology",

issn = "2079-7737",

publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",

number = "3",

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TY - JOUR

T1 - miR-4432 Targets FGFBP1 in Human Endothelial Cells

AU - Avvisato, Roberta

AU - Mone, Pasquale

AU - Jankauskas, Stanislovas S.

AU - Varzideh, Fahimeh

AU - Kansakar, Urna

AU - Gambardella, Jessica

AU - De Luca, Antonio

AU - Matarese, Alessandro

AU - Santulli, Gaetano

PY - 2023/3

Y1 - 2023/3

N2 - MicroRNAs (miRs) are small non-coding RNAs that modulate the expression of several target genes. Fibroblast growth factor binding protein 1 (FGFBP1) has been associated with endothelial dysfunction at the level of the blood–brain barrier (BBB). However, the underlying mechanisms are mostly unknown and there are no studies investigating the relationship between miRs and FGFBP1. Thus, the overarching aim of the present study was to identify and validate which miR can specifically target FGFBP1 in human brain microvascular endothelial cells, which represent the best in vitro model of the BBB. We were able to identify and validate miR-4432 as a fundamental modulator of FGFBP1 and we demonstrated that miR-4432 significantly reduces mitochondrial oxidative stress, a well-established pathophysiological hallmark of hypertension.

AB - MicroRNAs (miRs) are small non-coding RNAs that modulate the expression of several target genes. Fibroblast growth factor binding protein 1 (FGFBP1) has been associated with endothelial dysfunction at the level of the blood–brain barrier (BBB). However, the underlying mechanisms are mostly unknown and there are no studies investigating the relationship between miRs and FGFBP1. Thus, the overarching aim of the present study was to identify and validate which miR can specifically target FGFBP1 in human brain microvascular endothelial cells, which represent the best in vitro model of the BBB. We were able to identify and validate miR-4432 as a fundamental modulator of FGFBP1 and we demonstrated that miR-4432 significantly reduces mitochondrial oxidative stress, a well-established pathophysiological hallmark of hypertension.

KW - HUVEC

KW - blood pressure

KW - blood–brain barrier

KW - cerebrovascular disease

KW - endothelial dysfunction

KW - hBMECs

KW - hypertension

KW - miR-4432-3p

KW - miRNA

KW - microRNA

UR - http://www.scopus.com/inward/record.url?scp=85152714361&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85152714361&partnerID=8YFLogxK

U2 - 10.3390/biology12030459

DO - 10.3390/biology12030459

M3 - Article

AN - SCOPUS:85152714361

SN - 2079-7737

VL - 12

JO - Biology

JF - Biology

IS - 3

M1 - 459

ER -

miR-4432 Targets FGFBP1 in Human Endothelial Cells

Abstract

Keywords

ASJC Scopus subject areas

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