Microarray gene expression profiling of angiogenesis inhibitors using the rat aortic ring assay

T. G. Zogakis; N. G. Costouros; E. A. Kruger; S. Forbes; M. He; M. Qian; A. L. Feldman; W. D. Figg; H. R. Alexander; E. T. Liu; E. C. Kohn; Steven K. Libutti

doi:10.2144/02333dd02_11820a

Microarray gene expression profiling of angiogenesis inhibitors using the rat aortic ring assay

T. G. Zogakis, N. G. Costouros, E. A. Kruger, S. Forbes, M. He, M. Qian, A. L. Feldman, W. D. Figg, H. R. Alexander, E. T. Liu, E. C. Kohn, Steven K. Libutti

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20 Scopus citations

Abstract

The rat aortic ring assay has been previously described as a useful ex vivo model for analyzing the biological activity of various inhibitors of angiogenesis. Rat aortic rings are exposed to antiangiogenic agents for a five-day incubation period. Then, the degree of microvessel outgrowth from the rings is analyzed and quantified. In contrast to most in vitro angiogenesis assays, the rat aortic ring model provides a unique microenvironment to evaluate the interaction of various cell types and biological factors for their influence on angiogenesis. Microarray analysis is an accepted method for the evaluation of gene expression profiles and can be used to better understand changes in gene expression that occur when rat aortic rings are exposed to a particular biological agent. Here we describe a method of using microarray technology to evaluate the modulation of gene expression in angiogenesis using the rat aortic ring assay.

Original language	English (US)
Pages (from-to)	664-670
Number of pages	7
Journal	BioTechniques
Volume	33
Issue number	3
DOIs	https://doi.org/10.2144/02333dd02_11820a
State	Published - Sep 2002
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
General Biochemistry, Genetics and Molecular Biology

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title = "Microarray gene expression profiling of angiogenesis inhibitors using the rat aortic ring assay",

abstract = "The rat aortic ring assay has been previously described as a useful ex vivo model for analyzing the biological activity of various inhibitors of angiogenesis. Rat aortic rings are exposed to antiangiogenic agents for a five-day incubation period. Then, the degree of microvessel outgrowth from the rings is analyzed and quantified. In contrast to most in vitro angiogenesis assays, the rat aortic ring model provides a unique microenvironment to evaluate the interaction of various cell types and biological factors for their influence on angiogenesis. Microarray analysis is an accepted method for the evaluation of gene expression profiles and can be used to better understand changes in gene expression that occur when rat aortic rings are exposed to a particular biological agent. Here we describe a method of using microarray technology to evaluate the modulation of gene expression in angiogenesis using the rat aortic ring assay.",

author = "Zogakis, {T. G.} and Costouros, {N. G.} and Kruger, {E. A.} and S. Forbes and M. He and M. Qian and Feldman, {A. L.} and Figg, {W. D.} and Alexander, {H. R.} and Liu, {E. T.} and Kohn, {E. C.} and Libutti, {Steven K.}",

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AU - Zogakis, T. G.

AU - Costouros, N. G.

AU - Kruger, E. A.

AU - Forbes, S.

AU - He, M.

AU - Qian, M.

AU - Feldman, A. L.

AU - Figg, W. D.

AU - Alexander, H. R.

AU - Liu, E. T.

AU - Kohn, E. C.

AU - Libutti, Steven K.

PY - 2002/9

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AB - The rat aortic ring assay has been previously described as a useful ex vivo model for analyzing the biological activity of various inhibitors of angiogenesis. Rat aortic rings are exposed to antiangiogenic agents for a five-day incubation period. Then, the degree of microvessel outgrowth from the rings is analyzed and quantified. In contrast to most in vitro angiogenesis assays, the rat aortic ring model provides a unique microenvironment to evaluate the interaction of various cell types and biological factors for their influence on angiogenesis. Microarray analysis is an accepted method for the evaluation of gene expression profiles and can be used to better understand changes in gene expression that occur when rat aortic rings are exposed to a particular biological agent. Here we describe a method of using microarray technology to evaluate the modulation of gene expression in angiogenesis using the rat aortic ring assay.

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Microarray gene expression profiling of angiogenesis inhibitors using the rat aortic ring assay

Abstract

ASJC Scopus subject areas

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