Methodological standards for in vitro models of epilepsy and epileptic seizures. A TASK1-WG4 report of the AES/ILAE Translational Task Force of the ILAE

Joseph V. Raimondo; Uwe Heinemann; Marco de Curtis; Howard P. Goodkin; Chris G. Dulla; Damir Janigro; Akio Ikeda; Chou Ching K. Lin; Premysl Jiruska; Aristea S. Galanopoulou; Christophe Bernard

doi:10.1111/epi.13901

Methodological standards for in vitro models of epilepsy and epileptic seizures. A TASK1-WG4 report of the AES/ILAE Translational Task Force of the ILAE

Joseph V. Raimondo, Uwe Heinemann, Marco de Curtis, Howard P. Goodkin, Chris G. Dulla, Damir Janigro, Akio Ikeda, Chou Ching K. Lin, Premysl Jiruska, Aristea S. Galanopoulou, Christophe Bernard

Neurology

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

In vitro preparations are a powerful tool to explore the mechanisms and processes underlying epileptogenesis and ictogenesis. In this review, we critically review the numerous in vitro methodologies utilized in epilepsy research. We provide support for the inclusion of detailed descriptions of techniques, including often ignored parameters with unpredictable yet significant effects on study reproducibility and outcomes. In addition, we explore how recent developments in brain slice preparation relate to their use as models of epileptic activity.

Original language	English (US)
Pages (from-to)	40-52
Number of pages	13
Journal	Epilepsia
Volume	58
DOIs	https://doi.org/10.1111/epi.13901
State	Published - Nov 2017

Keywords

Animal selection and killing
Brain slice preparation
Electrophysiological recording methods
In vitro models of seizures
Recording solution composition

ASJC Scopus subject areas

Neurology
Clinical Neurology

Access to Document

10.1111/epi.13901

Cite this

Raimondo, J. V., Heinemann, U., de Curtis, M., Goodkin, H. P., Dulla, C. G., Janigro, D., Ikeda, A., Lin, C. C. K., Jiruska, P., Galanopoulou, A. S., & Bernard, C. (2017). Methodological standards for in vitro models of epilepsy and epileptic seizures. A TASK1-WG4 report of the AES/ILAE Translational Task Force of the ILAE. Epilepsia, 58, 40-52. https://doi.org/10.1111/epi.13901

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abstract = "In vitro preparations are a powerful tool to explore the mechanisms and processes underlying epileptogenesis and ictogenesis. In this review, we critically review the numerous in vitro methodologies utilized in epilepsy research. We provide support for the inclusion of detailed descriptions of techniques, including often ignored parameters with unpredictable yet significant effects on study reproducibility and outcomes. In addition, we explore how recent developments in brain slice preparation relate to their use as models of epileptic activity.",

keywords = "Animal selection and killing, Brain slice preparation, Electrophysiological recording methods, In vitro models of seizures, Recording solution composition",

author = "Raimondo, {Joseph V.} and Uwe Heinemann and {de Curtis}, Marco and Goodkin, {Howard P.} and Dulla, {Chris G.} and Damir Janigro and Akio Ikeda and Lin, {Chou Ching K.} and Premysl Jiruska and Galanopoulou, {Aristea S.} and Christophe Bernard",

note = "Publisher Copyright: Wiley Periodicals, Inc. {\textcopyright} 2017 International League Against Epilepsy",

year = "2017",

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doi = "10.1111/epi.13901",

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AU - de Curtis, Marco

AU - Goodkin, Howard P.

AU - Dulla, Chris G.

AU - Janigro, Damir

AU - Ikeda, Akio

AU - Lin, Chou Ching K.

AU - Jiruska, Premysl

AU - Galanopoulou, Aristea S.

AU - Bernard, Christophe

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