Messenger RNA levels and methlation patterns of GAPDH and β-actin genes in rat liver, spleen and brain in relation to aging

P. Eline Slagboom; Wiljo J.F. De Leeuw; Jan Vijg

doi:10.1016/0047-6374(90)90042-E

Messenger RNA levels and methlation patterns of GAPDH and β-actin genes in rat liver, spleen and brain in relation to aging

P. Eline Slagboom
, Wiljo J.F. De Leeuw
, Jan Vijg

Research output: Contribution to journal › Article › peer-review

66 Scopus citations

Abstract

Messenger RNA levels and methylation patterns of the glyceraldehyde-3-phosphate-dehydrogenase (GAPHD) and β-actin genes were studied in spleen, liver and brain of 6-, 24- and 36-month old female inbred rats. In the spleen, the mRNA levels of both housekeeping genes significantly increased between 24 and 36 months. No age-related alterations in the expression of GAPDH or β-actin mRNA were observed in brain or liver. A considerable intertissue and interindividual variation was observed in the mRNA levels of these genes in all age-groups as compared to the level of 28 S rRNA, which was used as an internal control. In this respect the interindividual variation in the level of GAPDH mRNA paralleled the variation observed in the β-actin mRNA level in the three tissues studied. The methylation pattern of β-actin was found to be tissue-specific in contrast to that of GAPDH, which was identical in all three tissues. No significant age-related alterations were observed in the GAPDH methylation pattern, whereas β-actin appeared to become slightly demethylated with age in the spleen at the CpG site for which tissue-specificity was observed.

Original language	English (US)
Pages (from-to)	243-257
Number of pages	15
Journal	Mechanisms of Ageing and Development
Volume	53
Issue number	3
DOIs	https://doi.org/10.1016/0047-6374(90)90042-E
State	Published - Apr 30 1990
Externally published	Yes

Keywords

Aging
DNA methylation
GAPDH
Gene expression
mRNA
β-Actin

ASJC Scopus subject areas

Aging
Developmental Biology

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10.1016/0047-6374(90)90042-E

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@article{5eb84b02483c4cdfad20dbb6bfaa8689,

title = "Messenger RNA levels and methlation patterns of GAPDH and β-actin genes in rat liver, spleen and brain in relation to aging",

abstract = "Messenger RNA levels and methylation patterns of the glyceraldehyde-3-phosphate-dehydrogenase (GAPHD) and β-actin genes were studied in spleen, liver and brain of 6-, 24- and 36-month old female inbred rats. In the spleen, the mRNA levels of both housekeeping genes significantly increased between 24 and 36 months. No age-related alterations in the expression of GAPDH or β-actin mRNA were observed in brain or liver. A considerable intertissue and interindividual variation was observed in the mRNA levels of these genes in all age-groups as compared to the level of 28 S rRNA, which was used as an internal control. In this respect the interindividual variation in the level of GAPDH mRNA paralleled the variation observed in the β-actin mRNA level in the three tissues studied. The methylation pattern of β-actin was found to be tissue-specific in contrast to that of GAPDH, which was identical in all three tissues. No significant age-related alterations were observed in the GAPDH methylation pattern, whereas β-actin appeared to become slightly demethylated with age in the spleen at the CpG site for which tissue-specificity was observed.",

keywords = "Aging, DNA methylation, GAPDH, Gene expression, mRNA, β-Actin",

author = "Slagboom, \{P. Eline\} and \{De Leeuw\}, \{Wiljo J.F.\} and Jan Vijg",

note = "Funding Information: This research was supported by the Netherlands Organization for Advancement of Pure Research (NWO) and by NATO (grant No. 86/0668) We thank Dr. C. Zurcher for histopathological analysis and helpful discussions. We thank Prof. Dr. P.L. Pearson and Dr. A.G. Uitterlinden for helpful disussions and A.A. Glaudemans for preparation of the photographs. We also thank Dr. M. Piechaczyk for kindly providing the GAPDH probe, Dr. B.D. Young for the 28 S rDNA probe and Dr. F. Ramaekers for providing the/3-actin probe.",

year = "1990",

month = apr,

day = "30",

doi = "10.1016/0047-6374(90)90042-E",

language = "English (US)",

volume = "53",

pages = "243--257",

journal = "Mechanisms of Ageing and Development",

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TY - JOUR

T1 - Messenger RNA levels and methlation patterns of GAPDH and β-actin genes in rat liver, spleen and brain in relation to aging

AU - Slagboom, P. Eline

AU - De Leeuw, Wiljo J.F.

AU - Vijg, Jan

N1 - Funding Information: This research was supported by the Netherlands Organization for Advancement of Pure Research (NWO) and by NATO (grant No. 86/0668) We thank Dr. C. Zurcher for histopathological analysis and helpful discussions. We thank Prof. Dr. P.L. Pearson and Dr. A.G. Uitterlinden for helpful disussions and A.A. Glaudemans for preparation of the photographs. We also thank Dr. M. Piechaczyk for kindly providing the GAPDH probe, Dr. B.D. Young for the 28 S rDNA probe and Dr. F. Ramaekers for providing the/3-actin probe.

PY - 1990/4/30

Y1 - 1990/4/30

N2 - Messenger RNA levels and methylation patterns of the glyceraldehyde-3-phosphate-dehydrogenase (GAPHD) and β-actin genes were studied in spleen, liver and brain of 6-, 24- and 36-month old female inbred rats. In the spleen, the mRNA levels of both housekeeping genes significantly increased between 24 and 36 months. No age-related alterations in the expression of GAPDH or β-actin mRNA were observed in brain or liver. A considerable intertissue and interindividual variation was observed in the mRNA levels of these genes in all age-groups as compared to the level of 28 S rRNA, which was used as an internal control. In this respect the interindividual variation in the level of GAPDH mRNA paralleled the variation observed in the β-actin mRNA level in the three tissues studied. The methylation pattern of β-actin was found to be tissue-specific in contrast to that of GAPDH, which was identical in all three tissues. No significant age-related alterations were observed in the GAPDH methylation pattern, whereas β-actin appeared to become slightly demethylated with age in the spleen at the CpG site for which tissue-specificity was observed.

AB - Messenger RNA levels and methylation patterns of the glyceraldehyde-3-phosphate-dehydrogenase (GAPHD) and β-actin genes were studied in spleen, liver and brain of 6-, 24- and 36-month old female inbred rats. In the spleen, the mRNA levels of both housekeeping genes significantly increased between 24 and 36 months. No age-related alterations in the expression of GAPDH or β-actin mRNA were observed in brain or liver. A considerable intertissue and interindividual variation was observed in the mRNA levels of these genes in all age-groups as compared to the level of 28 S rRNA, which was used as an internal control. In this respect the interindividual variation in the level of GAPDH mRNA paralleled the variation observed in the β-actin mRNA level in the three tissues studied. The methylation pattern of β-actin was found to be tissue-specific in contrast to that of GAPDH, which was identical in all three tissues. No significant age-related alterations were observed in the GAPDH methylation pattern, whereas β-actin appeared to become slightly demethylated with age in the spleen at the CpG site for which tissue-specificity was observed.

KW - Aging

KW - DNA methylation

KW - GAPDH

KW - Gene expression

KW - mRNA

KW - β-Actin

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SN - 0047-6374

VL - 53

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EP - 257

JO - Mechanisms of Ageing and Development

JF - Mechanisms of Ageing and Development

IS - 3

ER -

Messenger RNA levels and methlation patterns of GAPDH and β-actin genes in rat liver, spleen and brain in relation to aging

Abstract

Keywords

ASJC Scopus subject areas

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