@article{a2f125788d30418181148ed30fe7c387,
title = "Media matters! Alterations in the loading and release of Histoplasma capsulatum extracellular vesicles in response to different nutritional milieus",
abstract = "Histoplasma capsulatum is a dimorphic fungus that most frequently causes pneumonia, but can also disseminate and proliferate in diverse tissues. Histoplasma capsulatum has a complex secretion system that mediates the release of macromolecule-degrading enzymes and virulence factors. The formation and release of extracellular vesicles (EVs) are an important mechanism for non-conventional secretion in both ascomycetes and basidiomycetes. Histoplasma capsulatum EVs contain diverse proteins associated with virulence and are immunologically active. Despite the growing knowledge of EVs from H. capsulatum and other pathogenic fungi, the extent that changes in the environment impact the sorting of organic molecules in EVs has not been investigated. In this study, we cultivated H. capsulatum with distinct culture media to investigate the potential plasticity in EV loading in response to differences in nutrition. Our findings reveal that nutrition plays an important role in EV loading and formation, which may translate into differences in biological activities of these fungi in various fluids and tissues.",
keywords = "BHI, Ham's F12, Histoplasma capsulatum, RPMI, USA, culture media, dimorphic fungus, extracellular vesicles, histoplasmosis, lipidomics, metabolomics, proteomics",
author = "Cleare, {Levi G.} and Daniel Zamith and Heyman, {Heino M.} and Couvillion, {Sneha P.} and Leonardo Nimrichter and Rodrigues, {Marcio L.} and Nakayasu, {Ernesto S.} and Nosanchuk, {Joshua D.}",
note = "Funding Information: J.D.N. was supported in part by NIH R01AI052733 and R21AI124797. E.S.N. was partially supported by NIH R21AI124797. Parts of this work were performed in the Environmental Molecular Science Laboratory, a U.S. Department of Energy (DOE) national scientific user facility at Pacific Northwest National Laboratory (PNNL) in Richland, WA. MLR and LN are supported by grants from the Brazilian agency Conselho Nacional de Desenvolvimento Cient{\'i}fico e Tecnol{\'o}gico (CNPq, grants 405520/2018-2, 440015/2018-9, and 301304/2017-3 to M.L.R.; 311179/2017-7 and 408711/2017-7 to L.N.) and Fiocruz (grants VPPCB-007-FIO-18-2-57 and VPPIS-001-FIO-18-66 to M.L.R,). We also acknowledge support from the Instituto Nacional de Ci{\^e}ncia e Tecnologia de Inova{\c c}{\~a}o em Doen{\c c}as de Popula{\c c}{\~o}es Negligenciadas (INCT-IDPN). M.L.R. is currently on leave from the position of Associate Professor at the Microbiology Institute of the Federal University of Rio de Janeiro, Brazil. Funding Information: Conselho Nacional de Desenvolvimento Cient{\'i}fico e Tecnol{\'o}gico, Grant/Award Numbers: 301304/2017‐3, 311179/2017‐7, 405520/2018‐2, 408711/2017‐7, 440015/2018‐9; Foundation for the National Institutes of Health, Grant/Award Numbers: R01AI052733, R21AI124797; Funda{\c c}{\~a}o Oswaldo Cruz, Grant/Award Numbers: VPPCB‐007‐FIO‐18‐2‐57, VPPIS‐001‐FIO‐18‐66 Funding information Funding Information: J.D.N. was supported in part by NIH R01AI052733 and R21AI124797. E.S.N. was partially supported by NIH R21AI124797. Parts of this work were performed in the Environmental Molecular Science Laboratory, a U.S. Department of Energy (DOE) national scientific user facility at Pacific Northwest National Laboratory (PNNL) in Richland, WA. MLR and LN are supported by grants from the Brazilian agency Conselho Nacional de Desenvolvimento Cient{\'i}fico e Tecnol{\'o}gico (CNPq, grants 405520/2018‐2, 440015/2018‐9, and 301304/2017‐3 to M.L.R.; 311179/2017‐7 and 408711/2017‐7 to L.N.) and Fiocruz (grants VPPCB‐007‐FIO‐18‐2‐57 and VPPIS‐001‐FIO‐18‐66 to M.L.R,). We also acknowledge support from the Instituto Nacional de Ci{\^e}ncia e Tecnologia de Inova{\c c}{\~a}o em Doen{\c c}as de Popula{\c c}{\~o}es Negligenciadas (INCT‐IDPN). M.L.R. is currently on leave from the position of Associate Professor at the Microbiology Institute of the Federal University of Rio de Janeiro, Brazil. Publisher Copyright: {\textcopyright} 2020 John Wiley & Sons Ltd",
year = "2020",
month = sep,
day = "1",
doi = "10.1111/cmi.13217",
language = "English (US)",
volume = "22",
journal = "Cellular Microbiology",
issn = "1462-5814",
publisher = "Wiley-Blackwell",
number = "9",
}