TY - JOUR
T1 - Manganese alters rat brain amino acids levels
AU - Santos, Dinamene
AU - Batoreu, M. Camila
AU - Almeida, Isabel
AU - Ramos, Ruben
AU - Sidoryk-Wegrzynowicz, M.
AU - Aschner, Michael
AU - Marreilha Dos Santos, A. P.
N1 - Funding Information:
Acknowledgments This study was funded by FCT (Foundation for Science and Technology of Portugal; SFRH/BD/64128/2009), by i-Med.UL, Faculty of Pharmacy, University of Lisbon and a grant from the National Institute of Environmental Health Sciences ES R01 10563 (MA).
PY - 2012/12
Y1 - 2012/12
N2 - Manganese (Mn) is an essential element and it acts as a cofactor for a number of enzymatic reactions, including those involved in amino acid, lipid, protein, and carbohydrate metabolism. Excessive exposure to Mn can lead to poisoning, characterized by psychiatric disturbances and an extrapyramidal disorder. Mn-induced neuronal degeneration is associated with alterations in amino acids metabolism. In the present study, we analyzed whole rat brain amino acid content subsequent to four or eight intraperitoneal injections, with 25 mg MnCl2/kg/day, at 48-h intervals. We noted a significant increase in glycine brain levels after four or eight Mn injections (p < 0.05 and p < 0.01, respectively) and arginine also after four or eight injections (p < 0.001). Significant increases were also noted in brain proline (p < 0.01), cysteine (p < 0.05), phenylalanine (p < 0.01), and tyrosine (p < 0.01) levels after eight Mn injections vs. the control group. These findings suggest that Mn-induced alterations in amino acid levels secondary to Mn affect the neurochemical milieu.
AB - Manganese (Mn) is an essential element and it acts as a cofactor for a number of enzymatic reactions, including those involved in amino acid, lipid, protein, and carbohydrate metabolism. Excessive exposure to Mn can lead to poisoning, characterized by psychiatric disturbances and an extrapyramidal disorder. Mn-induced neuronal degeneration is associated with alterations in amino acids metabolism. In the present study, we analyzed whole rat brain amino acid content subsequent to four or eight intraperitoneal injections, with 25 mg MnCl2/kg/day, at 48-h intervals. We noted a significant increase in glycine brain levels after four or eight Mn injections (p < 0.05 and p < 0.01, respectively) and arginine also after four or eight injections (p < 0.001). Significant increases were also noted in brain proline (p < 0.01), cysteine (p < 0.05), phenylalanine (p < 0.01), and tyrosine (p < 0.01) levels after eight Mn injections vs. the control group. These findings suggest that Mn-induced alterations in amino acid levels secondary to Mn affect the neurochemical milieu.
KW - Amino acids
KW - Brain
KW - Glutamate
KW - Manganese
KW - Neurotoxicity
KW - γ-Aminobutyric acid (GABA)
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U2 - 10.1007/s12011-012-9504-8
DO - 10.1007/s12011-012-9504-8
M3 - Article
C2 - 22971893
AN - SCOPUS:84870700122
SN - 0163-4984
VL - 150
SP - 337
EP - 341
JO - Biological Trace Element Research
JF - Biological Trace Element Research
IS - 1-3
ER -
