Macrophages promote collagen fibrillogenesis around terminal end buds of the developing mammary gland

Wendy V. Ingman, Jeff Wyckoff, Valerie Gouon-Evans, John Condeelis, Jeffrey W. Pollard

Research output: Contribution to journalArticlepeer-review

210 Scopus citations


Development of the ductal network in the mammary gland is dependent in part on the presence of macrophages. Here we utilize multi-photon microscopy and second harmonic generation to describe terminal end bud 3-dimensional structure and the organization of the surrounding collagen matrix. We have applied this approach to analyze the effect of macrophage deficiency on terminal end bud structure and collagen organization, using mice homozygous for a null mutation in the colony stimulating factor-1 gene (Csf1op/Csf1op). Primary terminal end buds have an oblong shape, with long collagen I fibers close to the neck of the terminal end bud and radiating upwards in the direction of growth. Around the terminal end buds, the amount of total collagen I detected by antibody staining was not affected by macrophage deficiency. However the amount of collagen I organized into long fibers, detected by second harmonic generation signal, was reduced in Csf1op/Csf1op mice. Macrophage deficiency also caused terminal end buds to be rounder and shorter. These studies reveal a role for macrophages in collagen fibrillogenesis and in organization of the structure of terminal end buds.

Original languageEnglish (US)
Pages (from-to)3222-3229
Number of pages8
JournalDevelopmental Dynamics
Issue number12
StatePublished - Dec 2006


  • Collagen
  • Development
  • Macrophage
  • Mammary gland
  • Multi-photon microscope

ASJC Scopus subject areas

  • Developmental Biology


Dive into the research topics of 'Macrophages promote collagen fibrillogenesis around terminal end buds of the developing mammary gland'. Together they form a unique fingerprint.

Cite this