LspA-independent action of globomycin on Mycobacterium tuberculosis

Niaz Banaiee; William R. Jacobs; Joel D. Ernst

doi:10.1093/jac/dkm223

LspA-independent action of globomycin on Mycobacterium tuberculosis

Niaz Banaiee, William R. Jacobs, Joel D. Ernst

Microbiology & Immunology

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Objectives: The objective of this study was to investigate the antimicrobial activity and specificity of globomycin, an inhibitor of lipoprotein signal peptidase II (LspA), against Mycobacterium tuberculosis. Methods: The mycobactericidal and mycobacteriostatic activity of globomycin was determined by optical density and cfu plating. The specificity of globomycin was determined by western immunoblotting using anti-MPT83 antibody. Results: Globomycin is mycobactericidal at concentrations ≥40 mg/L. However, at 80 mg/L, the processing of the lipoprotein MPT-83 is unaffected and growth-inhibitory effect of globomycin is unchanged in an lspA null mutant (Δ lspA:: lspA_mut) lacking the putative drug target. Conclusions: Globomycin kills M. tuberculosis through a mechanism that is independent of LspA.

Original language	English (US)
Pages (from-to)	414-416
Number of pages	3
Journal	Journal of Antimicrobial Chemotherapy
Volume	60
Issue number	2
DOIs	https://doi.org/10.1093/jac/dkm223
State	Published - Aug 2007

Keywords

Antimycobacterials
Lipoprotein processing
Lipoprotein signal peptidase
Lipoproteins

ASJC Scopus subject areas

Pharmacology
Microbiology (medical)
Infectious Diseases
Pharmacology (medical)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1093/jac/dkm223

Cite this

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title = "LspA-independent action of globomycin on Mycobacterium tuberculosis",

abstract = "Objectives: The objective of this study was to investigate the antimicrobial activity and specificity of globomycin, an inhibitor of lipoprotein signal peptidase II (LspA), against Mycobacterium tuberculosis. Methods: The mycobactericidal and mycobacteriostatic activity of globomycin was determined by optical density and cfu plating. The specificity of globomycin was determined by western immunoblotting using anti-MPT83 antibody. Results: Globomycin is mycobactericidal at concentrations ≥40 mg/L. However, at 80 mg/L, the processing of the lipoprotein MPT-83 is unaffected and growth-inhibitory effect of globomycin is unchanged in an lspA null mutant (Δ lspA:: lspAmut) lacking the putative drug target. Conclusions: Globomycin kills M. tuberculosis through a mechanism that is independent of LspA.",

keywords = "Antimycobacterials, Lipoprotein processing, Lipoprotein signal peptidase, Lipoproteins",

author = "Niaz Banaiee and Jacobs, {William R.} and Ernst, {Joel D.}",

note = "Funding Information: This work was supported by the National Institutes of Health grants R01 AI46097 and K08 AI061105.",

year = "2007",

month = aug,

doi = "10.1093/jac/dkm223",

language = "English (US)",

volume = "60",

pages = "414--416",

journal = "Journal of Antimicrobial Chemotherapy",

issn = "0305-7453",

publisher = "Oxford University Press",

number = "2",

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TY - JOUR

T1 - LspA-independent action of globomycin on Mycobacterium tuberculosis

AU - Banaiee, Niaz

AU - Jacobs, William R.

AU - Ernst, Joel D.

N1 - Funding Information: This work was supported by the National Institutes of Health grants R01 AI46097 and K08 AI061105.

PY - 2007/8

Y1 - 2007/8

N2 - Objectives: The objective of this study was to investigate the antimicrobial activity and specificity of globomycin, an inhibitor of lipoprotein signal peptidase II (LspA), against Mycobacterium tuberculosis. Methods: The mycobactericidal and mycobacteriostatic activity of globomycin was determined by optical density and cfu plating. The specificity of globomycin was determined by western immunoblotting using anti-MPT83 antibody. Results: Globomycin is mycobactericidal at concentrations ≥40 mg/L. However, at 80 mg/L, the processing of the lipoprotein MPT-83 is unaffected and growth-inhibitory effect of globomycin is unchanged in an lspA null mutant (Δ lspA:: lspAmut) lacking the putative drug target. Conclusions: Globomycin kills M. tuberculosis through a mechanism that is independent of LspA.

AB - Objectives: The objective of this study was to investigate the antimicrobial activity and specificity of globomycin, an inhibitor of lipoprotein signal peptidase II (LspA), against Mycobacterium tuberculosis. Methods: The mycobactericidal and mycobacteriostatic activity of globomycin was determined by optical density and cfu plating. The specificity of globomycin was determined by western immunoblotting using anti-MPT83 antibody. Results: Globomycin is mycobactericidal at concentrations ≥40 mg/L. However, at 80 mg/L, the processing of the lipoprotein MPT-83 is unaffected and growth-inhibitory effect of globomycin is unchanged in an lspA null mutant (Δ lspA:: lspAmut) lacking the putative drug target. Conclusions: Globomycin kills M. tuberculosis through a mechanism that is independent of LspA.

KW - Antimycobacterials

KW - Lipoprotein processing

KW - Lipoprotein signal peptidase

KW - Lipoproteins

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JF - Journal of Antimicrobial Chemotherapy

IS - 2

ER -

LspA-independent action of globomycin on Mycobacterium tuberculosis

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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