Localization of plasma membrane and secretory calcium pumps in the mammary gland

Helen M. Faddy; Chanel E. Smart; Ren Xu; Genee Y. Lee; Paraic A. Kenny; Mingye Feng; Rajini Rao; Melissa A. Brown; Mina J. Bissell; Sarah J. Roberts-Thomson; Gregory R. Monteith

doi:10.1016/j.bbrc.2008.03.003

Localization of plasma membrane and secretory calcium pumps in the mammary gland

Helen M. Faddy, Chanel E. Smart, Ren Xu, Genee Y. Lee, Paraic A. Kenny, Mingye Feng, Rajini Rao, Melissa A. Brown, Mina J. Bissell, Sarah J. Roberts-Thomson, Gregory R. Monteith

Research output: Contribution to journal › Article › peer-review

71 Scopus citations

Abstract

Until recently the mechanism for the enrichment of milk with calcium was thought to be almost entirely via the secretory pathway. However, recent studies suggest that a plasma membrane calcium ATPase, PMCA2, is the primary mechanism for calcium transport into milk, highlighting a major role for apical calcium transport. We compared the expression of the recently identified secretory calcium ATPase, SPCA2, and SPCA1, in the mouse mammary gland during development. SPCA2 levels increased over 35-fold during lactation with expression localized to luminal secretory cells, while SPCA1 increased only a modest 2-fold and was expressed throughout the cells of the mammary gland. We also observed major differences in the localization of PMCA2 and PMCA1. Our studies highlight the likely specific roles of PMCA2 and SPCA2 in lactation and indicate that calcium transport into milk is a complex interplay between apical and secretory pathways.

Original language	English (US)
Pages (from-to)	977-981
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	369
Issue number	3
DOIs	https://doi.org/10.1016/j.bbrc.2008.03.003
State	Published - May 9 2008
Externally published	Yes

Keywords

3D mammary culture
Calcium
Mammary gland
Milk
Mouse
PMCA1
PMCA2
SPCA1
SPCA2

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2008.03.003

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title = "Localization of plasma membrane and secretory calcium pumps in the mammary gland",

abstract = "Until recently the mechanism for the enrichment of milk with calcium was thought to be almost entirely via the secretory pathway. However, recent studies suggest that a plasma membrane calcium ATPase, PMCA2, is the primary mechanism for calcium transport into milk, highlighting a major role for apical calcium transport. We compared the expression of the recently identified secretory calcium ATPase, SPCA2, and SPCA1, in the mouse mammary gland during development. SPCA2 levels increased over 35-fold during lactation with expression localized to luminal secretory cells, while SPCA1 increased only a modest 2-fold and was expressed throughout the cells of the mammary gland. We also observed major differences in the localization of PMCA2 and PMCA1. Our studies highlight the likely specific roles of PMCA2 and SPCA2 in lactation and indicate that calcium transport into milk is a complex interplay between apical and secretory pathways.",

keywords = "3D mammary culture, Calcium, Mammary gland, Milk, Mouse, PMCA1, PMCA2, SPCA1, SPCA2",

author = "Faddy, {Helen M.} and Smart, {Chanel E.} and Ren Xu and Lee, {Genee Y.} and Kenny, {Paraic A.} and Mingye Feng and Rajini Rao and Brown, {Melissa A.} and Bissell, {Mina J.} and Roberts-Thomson, {Sarah J.} and Monteith, {Gregory R.}",

note = "Funding Information: We wish to thank Prof. Malcolm Faddy for help with statistical analysis. This work was funded by a Australian Postgraduate Award (H.F.), a University of Queensland GSRTA (H.F.), a QLD Cancer Fund travel award (H.F.), and the University of Queensland Foundation, and by grants from the Office of Biological and Environmental Research of the US Department of Energy (DE-AC03-76SF00098 and a Distinguish Fellow Award to M.J.B.) and the National Cancer Institute (CA57621 to M.J.B. and Zena Werb). ",

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doi = "10.1016/j.bbrc.2008.03.003",

language = "English (US)",

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pages = "977--981",

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T1 - Localization of plasma membrane and secretory calcium pumps in the mammary gland

AU - Faddy, Helen M.

AU - Smart, Chanel E.

AU - Xu, Ren

AU - Lee, Genee Y.

AU - Kenny, Paraic A.

AU - Feng, Mingye

AU - Rao, Rajini

AU - Brown, Melissa A.

AU - Bissell, Mina J.

AU - Roberts-Thomson, Sarah J.

AU - Monteith, Gregory R.

N1 - Funding Information: We wish to thank Prof. Malcolm Faddy for help with statistical analysis. This work was funded by a Australian Postgraduate Award (H.F.), a University of Queensland GSRTA (H.F.), a QLD Cancer Fund travel award (H.F.), and the University of Queensland Foundation, and by grants from the Office of Biological and Environmental Research of the US Department of Energy (DE-AC03-76SF00098 and a Distinguish Fellow Award to M.J.B.) and the National Cancer Institute (CA57621 to M.J.B. and Zena Werb).

PY - 2008/5/9

Y1 - 2008/5/9

N2 - Until recently the mechanism for the enrichment of milk with calcium was thought to be almost entirely via the secretory pathway. However, recent studies suggest that a plasma membrane calcium ATPase, PMCA2, is the primary mechanism for calcium transport into milk, highlighting a major role for apical calcium transport. We compared the expression of the recently identified secretory calcium ATPase, SPCA2, and SPCA1, in the mouse mammary gland during development. SPCA2 levels increased over 35-fold during lactation with expression localized to luminal secretory cells, while SPCA1 increased only a modest 2-fold and was expressed throughout the cells of the mammary gland. We also observed major differences in the localization of PMCA2 and PMCA1. Our studies highlight the likely specific roles of PMCA2 and SPCA2 in lactation and indicate that calcium transport into milk is a complex interplay between apical and secretory pathways.

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KW - 3D mammary culture

KW - Calcium

KW - Mammary gland

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KW - Mouse

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KW - SPCA1

KW - SPCA2

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Localization of plasma membrane and secretory calcium pumps in the mammary gland

Abstract

Keywords

ASJC Scopus subject areas

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