Abstract
Differing spatial scales of signaling cascades are critical for cell orientation during chemotactic responses. We used biotin EGF bound to streptavidin-coupled magnetic beads to locally stimulate cells overexpressing the EGF receptor. We have found that EGF-induced actin polymerization remains localized even under conditions of receptor overexpression. Conversely, EGF-induced ERK activation spreads throughout the cell body after EGF bead stimulation. The localized actin polymerization is independent of PI3-kinase and rho protein activity and requires Arp2/3 complex and cofilin function. Thus, we find differing spatial scales of signaling from the EGF receptor, supporting models of chemotaxis that integrate short- and long-range signaling.
Original language | English (US) |
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Pages (from-to) | 781-787 |
Number of pages | 7 |
Journal | Journal of Cell Biology |
Volume | 162 |
Issue number | 5 |
DOIs | |
State | Published - Sep 1 2003 |
Keywords
- Chemotaxis
- EGF bead
- Epidermal growth factor
- Receptor
- Signal transduction
ASJC Scopus subject areas
- Cell Biology