Layer-specific blood-flow MRI of retinitis pigmentosa in RCS rats

Guang Li; Bryan De La Garza; Yen Yu I. Shih; Eric R. Muir; Timothy Q. Duong

doi:10.1016/j.exer.2012.06.006

Layer-specific blood-flow MRI of retinitis pigmentosa in RCS rats

Guang Li, Bryan De La Garza, Yen Yu I. Shih, Eric R. Muir, Timothy Q. Duong

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

The Royal College of Surgeons (RCS) rat is an established animal model of retinitis pigmentosa, a family of inherited retinal diseases which starts with loss of peripheral vision and progresses to eventual blindness. Blood flow (BF), an important physiological parameter, is intricately coupled to metabolic function under normal physiological conditions and is perturbed in many neurological and retinal diseases. This study reports non-invasive high-resolution MRI (44 × 44 × 600 μm) to image quantitative retinal and choroidal BF and layer-specific retinal thicknesses in RCS rat retinas at different stages of retinal degeneration compared with age-matched controls. The unique ability to separate retinal and choroidal BF was made possible by the depth-resolved MRI technique. RBF decreased with progressive retinal degeneration, but ChBF did not change in RCS rats up to post-natal day 90. We concluded that choroidal and retinal circulations have different susceptibility to progressive retinal degeneration in RCS rats. Layer-specific retinal thickness became progressively thinner and was corroborated by histological analysis in the same animals. MRI can detect progressive anatomical and BF changes during retinal degeneration with laminar resolution.

Original language	English (US)
Pages (from-to)	90-96
Number of pages	7
Journal	Experimental Eye Research
Volume	101
DOIs	https://doi.org/10.1016/j.exer.2012.06.006
State	Published - Aug 2012
Externally published	Yes

Keywords

Arterial spin labeling
Blood flow
Choroid
Retina
Retinal degeneration
Retinitis pigmentosa

ASJC Scopus subject areas

Ophthalmology
Sensory Systems
Cellular and Molecular Neuroscience

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10.1016/j.exer.2012.06.006

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title = "Layer-specific blood-flow MRI of retinitis pigmentosa in RCS rats",

abstract = "The Royal College of Surgeons (RCS) rat is an established animal model of retinitis pigmentosa, a family of inherited retinal diseases which starts with loss of peripheral vision and progresses to eventual blindness. Blood flow (BF), an important physiological parameter, is intricately coupled to metabolic function under normal physiological conditions and is perturbed in many neurological and retinal diseases. This study reports non-invasive high-resolution MRI (44 × 44 × 600 μm) to image quantitative retinal and choroidal BF and layer-specific retinal thicknesses in RCS rat retinas at different stages of retinal degeneration compared with age-matched controls. The unique ability to separate retinal and choroidal BF was made possible by the depth-resolved MRI technique. RBF decreased with progressive retinal degeneration, but ChBF did not change in RCS rats up to post-natal day 90. We concluded that choroidal and retinal circulations have different susceptibility to progressive retinal degeneration in RCS rats. Layer-specific retinal thickness became progressively thinner and was corroborated by histological analysis in the same animals. MRI can detect progressive anatomical and BF changes during retinal degeneration with laminar resolution.",

keywords = "Arterial spin labeling, Blood flow, Choroid, Retina, Retinal degeneration, Retinitis pigmentosa",

author = "Guang Li and {De La Garza}, Bryan and Shih, {Yen Yu I.} and Muir, {Eric R.} and Duong, {Timothy Q.}",

note = "Funding Information: Grant support: This work was supported in part by the NIH/NEI (R01 EY014211 and EY018855 ), and a MERIT Award from the Department of Veterans Affairs, and a Clinical Translational Science Award Supplement (parent grant UL1RR025767). ",

year = "2012",

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doi = "10.1016/j.exer.2012.06.006",

language = "English (US)",

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AU - Li, Guang

AU - De La Garza, Bryan

AU - Shih, Yen Yu I.

AU - Muir, Eric R.

AU - Duong, Timothy Q.

N1 - Funding Information: Grant support: This work was supported in part by the NIH/NEI (R01 EY014211 and EY018855 ), and a MERIT Award from the Department of Veterans Affairs, and a Clinical Translational Science Award Supplement (parent grant UL1RR025767).

PY - 2012/8

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N2 - The Royal College of Surgeons (RCS) rat is an established animal model of retinitis pigmentosa, a family of inherited retinal diseases which starts with loss of peripheral vision and progresses to eventual blindness. Blood flow (BF), an important physiological parameter, is intricately coupled to metabolic function under normal physiological conditions and is perturbed in many neurological and retinal diseases. This study reports non-invasive high-resolution MRI (44 × 44 × 600 μm) to image quantitative retinal and choroidal BF and layer-specific retinal thicknesses in RCS rat retinas at different stages of retinal degeneration compared with age-matched controls. The unique ability to separate retinal and choroidal BF was made possible by the depth-resolved MRI technique. RBF decreased with progressive retinal degeneration, but ChBF did not change in RCS rats up to post-natal day 90. We concluded that choroidal and retinal circulations have different susceptibility to progressive retinal degeneration in RCS rats. Layer-specific retinal thickness became progressively thinner and was corroborated by histological analysis in the same animals. MRI can detect progressive anatomical and BF changes during retinal degeneration with laminar resolution.

AB - The Royal College of Surgeons (RCS) rat is an established animal model of retinitis pigmentosa, a family of inherited retinal diseases which starts with loss of peripheral vision and progresses to eventual blindness. Blood flow (BF), an important physiological parameter, is intricately coupled to metabolic function under normal physiological conditions and is perturbed in many neurological and retinal diseases. This study reports non-invasive high-resolution MRI (44 × 44 × 600 μm) to image quantitative retinal and choroidal BF and layer-specific retinal thicknesses in RCS rat retinas at different stages of retinal degeneration compared with age-matched controls. The unique ability to separate retinal and choroidal BF was made possible by the depth-resolved MRI technique. RBF decreased with progressive retinal degeneration, but ChBF did not change in RCS rats up to post-natal day 90. We concluded that choroidal and retinal circulations have different susceptibility to progressive retinal degeneration in RCS rats. Layer-specific retinal thickness became progressively thinner and was corroborated by histological analysis in the same animals. MRI can detect progressive anatomical and BF changes during retinal degeneration with laminar resolution.

KW - Arterial spin labeling

KW - Blood flow

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KW - Retinal degeneration

KW - Retinitis pigmentosa

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Layer-specific blood-flow MRI of retinitis pigmentosa in RCS rats

Abstract

Keywords

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