TY - JOUR
T1 - L-tyrosine induces the production of a pyomelanin-like pigment by the parasitic yeast-form of Histoplasma capsulatum
AU - Almeida-Paes, Rodrigo
AU - Almeida-Silva, Fernando
AU - Pinto, Gabriela Costa Maia
AU - De Abreu Almeida, Marcos
AU - De Medeiros Muniz, Mauro
AU - Pizzini, Claudia Vera
AU - Gerfen, Gary J.
AU - Nosanchuk, Joshua Daniel
AU - Zancopé-Oliveira, Rosely Maria
N1 - Funding Information:
This work was supported by Conselho Nacional de desenvolvimento Científico e Tecnológico [grant number 449184/2014-5 to R. A.-P.]. R. A.-P. and R. M. Z.-O. are supported in part by Conselho Nacional de desenvolvimento Científico e Tecnológico [grant numbers 305487/2015-9, and 304976/2013-0, respectively]. J. D. N. is partially supported by NIH R21 AI124797 and AI052733.
Publisher Copyright:
© The Author 2017.
PY - 2018/6/1
Y1 - 2018/6/1
N2 - Melanization of Histoplasma capsulatum remains poorly described, particularly in regards to the forms of melanin produced. In the present study, 30 clinical and environmental H. capsulatum strains were grown in culture media with or without L-tyrosine under conditions that produced either mycelial or yeast forms. Mycelial cultures were not melanized under the studied conditions. However, all strains cultivated under yeast conditions produced a brownish to black soluble pigment compatible with pyomelanin when grew in presence of L-tyrosine. Sulcotrione inhibited pigment production in yeast cultures, strengthening the hyphothesis that H. capsulatum yeast forms produce pyomelanin. Since pyomelanin is produced by the fungal parasitic form, this pigment may be involved in H. capsulatum virulence.
AB - Melanization of Histoplasma capsulatum remains poorly described, particularly in regards to the forms of melanin produced. In the present study, 30 clinical and environmental H. capsulatum strains were grown in culture media with or without L-tyrosine under conditions that produced either mycelial or yeast forms. Mycelial cultures were not melanized under the studied conditions. However, all strains cultivated under yeast conditions produced a brownish to black soluble pigment compatible with pyomelanin when grew in presence of L-tyrosine. Sulcotrione inhibited pigment production in yeast cultures, strengthening the hyphothesis that H. capsulatum yeast forms produce pyomelanin. Since pyomelanin is produced by the fungal parasitic form, this pigment may be involved in H. capsulatum virulence.
KW - Histoplasma capsulatum
KW - L-tyrosine
KW - Pyomelanin
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U2 - 10.1093/mmy/myx068
DO - 10.1093/mmy/myx068
M3 - Article
C2 - 28992332
AN - SCOPUS:85055620139
SN - 1369-3786
VL - 56
SP - 506
EP - 509
JO - Medical mycology
JF - Medical mycology
IS - 4
ER -
