Isolation of neonatal rat cortical astrocytes for primary cultures.

J. W. Allen, L. A. Mutkus, M. Aschner

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


There is increasing interest in the role of astrocytes as mediators of neurotoxicity. This unit describes a method for preparing astrocyte cultures of greater than 95% purity by enzymatic dissociation from neonatal rat brain. These preparations have both high yield and high viability.

Original languageEnglish (US)
Pages (from-to)Unit12.4
JournalCurrent protocols in toxicology / editorial board, Mahin D. Maines (editor-in-chief) ... [et al.]
VolumeChapter 12
StatePublished - 2001
Externally publishedYes

ASJC Scopus subject areas

  • Toxicology


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