@article{1419b5e7f5d9497ba472ca7430e70347,
title = "Intravital imaging of metastatic behavior through a mammary imaging window",
abstract = "We report a technique to evaluate the same tumor microenvironment over multiple intravital imaging sessions in living mice. We optically marked individual tumor cells expressing photoswitchable proteins in an orthotopic mammary carcinoma and followed them for extended periods through a mammary imaging window. We found that two distinct microenvironments in the same orthotopic mammary tumor affected differently the invasion and intravasation of tumor cells.",
author = "Dmitriy Kedrin and Bojana Gligorijevic and Jeffrey Wyckoff and Verkhusha, {Vladislav V.} and John Condeelis and Segall, {Jeffrey E.} and {van Rheenen}, Jacco",
note = "Funding Information: This work was supported by US Department of Defense (BC061403 to D.K.), US National Institutes of Health (U54GM064346 to J.v.R.; CA100324 to J.C., J.E.S. and J.W.; U54CA126511 to J.C. and B.G.; and GM070358 and GM073913 to V.V.V.). We thank the staff of the Analytical Imaging Facility and D. Entenberg for help with microscopy, the immunohistochemistry facility for help with histology, M. Rottenkolber for help in fabrication of the imaging box, J. Pollard (Albert Einstein College of Medicine) for providing the F4/80 antibody, S. Garofalo for technical assistance, and members of the Condeelis, Segall, Cox and Verkhusha laboratories for discussions.",
year = "2008",
doi = "10.1038/nmeth.1269",
language = "English (US)",
volume = "5",
pages = "1019--1021",
journal = "Nature Methods",
issn = "1548-7091",
publisher = "Nature Publishing Group",
number = "12",
}