Abstract
In this chapter, we summarize and describe various methods that have been used to detect glycocalyx and to study the interactions between glycocalyx and actin cytoskeleton with the emphasis in the nervous tissue. We highlight two methodologies to analyze the direct interaction between glycocalyx and actin cytoskeleton: one method focusing on visualization using immunostaining and confocal microscopy techniques and the other focusing on direct protein-protein interactions using co-immunoprecipitation techniques.
| Original language | English (US) |
|---|---|
| Title of host publication | The Cytoskeleton |
| Subtitle of host publication | Imaging, Isolation, and Interaction |
| Publisher | Humana Press Inc. |
| Pages | 43-62 |
| Number of pages | 20 |
| ISBN (Print) | 9781627032650 |
| DOIs | |
| State | Published - 2013 |
Publication series
| Name | Neuromethods |
|---|---|
| Volume | 79 |
| ISSN (Print) | 0893-2336 |
| ISSN (Electronic) | 1940-6045 |
UN SDGs
This output contributes to the following UN Sustainable Development Goals (SDGs)
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SDG 3 Good Health and Well-being
Keywords
- Actin cytoskeleton
- Astrocyte
- Chondroitin sulfate
- Heparan sulfate
- Hyaluronic acid
ASJC Scopus subject areas
- Psychiatry and Mental health
- General Pharmacology, Toxicology and Pharmaceutics
- General Biochemistry, Genetics and Molecular Biology
- General Neuroscience
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