Abstract
Physiological concentrations of insulin stimulate the incorporation of 32P into ribosomal protein S6 in intact 3T3-L1 adipocytes. Extracts from cells treated with insulin also exhibit an increased ability to incorporate 32P from [γ-32P]ATP into protein S6 in vitro. The authors now report that the direct addition of insulin to particulate preparations containing high affinity insulin receptors and ribosomes derived from insulin-sensitive cells (not previously exposed to insulin) stimulates incorporation of 32P from [γ-32P]ATP into ribosomal protein S6 1.5- to 3.0-fold. The stimulation occurs at the same concentrations of insulin (0.1-1.0 nM) and within the same period of time as it does in intact cells.
Original language | English (US) |
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Pages (from-to) | 3630-3636 |
Number of pages | 7 |
Journal | Journal of Biological Chemistry |
Volume | 256 |
Issue number | 8 |
State | Published - 1981 |
Externally published | Yes |
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Cell Biology