Induction of Taurocholate Release from Isolated Rat Hepatocytes in Suspension by α‐Adrenergie Agents and Vasopressin: Implications for Control of Bile Salt Secretion

David A. Gewirtz, Joyce K. Randolph, I. David Goldman

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

Hepatocytes incubated with 25 μM. [3H]taurocholate rapidly deplete the extracellular medium of [3H]taurocholate and achieve a steady‐state level of intracellular bile salt within 15 min. Exposure of cells at steady state ith extracellular taurocholate to the catecholamines norepinephrine or epinephrine results in release of 3H from the cells into the incubation medium; the 3H released represents almost exclusively unmetabolized [3H]taurocholate. The hierarchy of effectiveness of the catecholamines, norepinephrine ≈ epinephrine > phenylephrine ≫ isoproterenol, is indicative of an α‐adrenergic mechanism. Induction of [3H]taurocholate release by norepinephrine is inhibited by the β‐antagonists phenoxybenzamine and phentolamine and by chlorpromazine, but is not affected by the β‐antagonist propranolol, further supporting an α‐adrenergic basis for this phenomenon. Arginine vasopressin, at concentrations of 1 ± 10−9 M and greater, also induces bile salt release. Classical α‐ and β‐antagonists have minimal effects on vasopressin induced bile salt release. While the peptide hormones angiotensin and oxytocin are, alone, relatively ineffective inducers of bile salt release, oxytocin potentiates the induction of bile salt release by vasopressin, suggesting complex interactions with membrane receptor function. Further studies assessing the interaction of sympathetic neurotransmitters and peptide hormones with bile salt transport and release in the hepatocyte may provide insight into the regulation of hepatic secretory function in the intact animal.

Original languageEnglish (US)
Pages (from-to)205-212
Number of pages8
JournalHepatology
Volume4
Issue number2
DOIs
StatePublished - 1984
Externally publishedYes

ASJC Scopus subject areas

  • Hepatology

Fingerprint

Dive into the research topics of 'Induction of Taurocholate Release from Isolated Rat Hepatocytes in Suspension by α‐Adrenergie Agents and Vasopressin: Implications for Control of Bile Salt Secretion'. Together they form a unique fingerprint.

Cite this