Increased phospholipase D activity in butyrate-induced differentiation of HT-29 cells

M. Madesh; O. Benard; K. A. Balasubramanian

doi:10.1016/S0304-3835(98)00172-4

Increased phospholipase D activity in butyrate-induced differentiation of HT-29 cells

M. Madesh, O. Benard, K. A. Balasubramanian

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3 Scopus citations

Abstract

Phospholipids are important constituents of biomembrane components and are supposed to function as enzyme activators or precursors of bioactive substances. Our earlier work has shown an increased esterification of neutral lipids of HT-29 cells during butyrate-induced differentiation (M. Madesh, O. Benard, K.A. Balasubramanian, Butyrate-induced alteration in lipid composition of human colon cell line HT-29, Biochem. Mol. Biol. Int. 38 (1996) 659-664). In this report we show that there is an increase in phospholipase D (PLD) activity during butyrate-induced differentiation of HT-29 cells as indicated by the formation of phosphatidic acid (PA). When the control and butyrate-treated cell homogenates were incubated in vitro with 1 mM Ca²⁺, the increase in PA formation was higher than in butyrate-treated cells. This PA was formed due to PLD activity that was confirmed by the generation of phosphatidylethanol by in vitro incubation of HT-29 cell homogenates in the presence of ethanol. The formation of PA was associated with a decrease in phosphatidylcholine (PC) and phosphatidylethanolamine (PE). This study has shown an increase in PLD activity associated with the differentiation of HT-29 cells. Copyright (C) 1998 Elsevier Science Ireland Ltd.

Original language	English (US)
Pages (from-to)	141-146
Number of pages	6
Journal	Cancer Letters
Volume	132
Issue number	1-2
DOIs	https://doi.org/10.1016/S0304-3835(98)00172-4
State	Published - Oct 23 1998
Externally published	Yes

Keywords

Butyrate
Differentiation
HT-29 cells
Phospholipase D

ASJC Scopus subject areas

Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S0304-3835(98)00172-4

Cite this

@article{e283f61ca8ed4216bbaad3b453781a6d,

title = "Increased phospholipase D activity in butyrate-induced differentiation of HT-29 cells",

abstract = "Phospholipids are important constituents of biomembrane components and are supposed to function as enzyme activators or precursors of bioactive substances. Our earlier work has shown an increased esterification of neutral lipids of HT-29 cells during butyrate-induced differentiation (M. Madesh, O. Benard, K.A. Balasubramanian, Butyrate-induced alteration in lipid composition of human colon cell line HT-29, Biochem. Mol. Biol. Int. 38 (1996) 659-664). In this report we show that there is an increase in phospholipase D (PLD) activity during butyrate-induced differentiation of HT-29 cells as indicated by the formation of phosphatidic acid (PA). When the control and butyrate-treated cell homogenates were incubated in vitro with 1 mM Ca2+, the increase in PA formation was higher than in butyrate-treated cells. This PA was formed due to PLD activity that was confirmed by the generation of phosphatidylethanol by in vitro incubation of HT-29 cell homogenates in the presence of ethanol. The formation of PA was associated with a decrease in phosphatidylcholine (PC) and phosphatidylethanolamine (PE). This study has shown an increase in PLD activity associated with the differentiation of HT-29 cells. Copyright (C) 1998 Elsevier Science Ireland Ltd.",

keywords = "Butyrate, Differentiation, HT-29 cells, Phospholipase D",

author = "M. Madesh and O. Benard and Balasubramanian, {K. A.}",

note = "Funding Information: The Wellcome Trust Research Laboratory is supported by the Wellcome Trust, London. Financial assistance from the Department of Science and Technology and Indian Council of Medical Research, Government of India is gratefully acknowledged. M.M. is a Senior Research Fellow and O.B. is a Research Associate of the Council of Scientific and Industrial Research, India. The authors thank Mr G. Subramani for his help in cell culture work.",

year = "1998",

month = oct,

day = "23",

doi = "10.1016/S0304-3835(98)00172-4",

language = "English (US)",

volume = "132",

pages = "141--146",

journal = "Cancer Letters",

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TY - JOUR

T1 - Increased phospholipase D activity in butyrate-induced differentiation of HT-29 cells

AU - Madesh, M.

AU - Benard, O.

AU - Balasubramanian, K. A.

N1 - Funding Information: The Wellcome Trust Research Laboratory is supported by the Wellcome Trust, London. Financial assistance from the Department of Science and Technology and Indian Council of Medical Research, Government of India is gratefully acknowledged. M.M. is a Senior Research Fellow and O.B. is a Research Associate of the Council of Scientific and Industrial Research, India. The authors thank Mr G. Subramani for his help in cell culture work.

PY - 1998/10/23

Y1 - 1998/10/23

N2 - Phospholipids are important constituents of biomembrane components and are supposed to function as enzyme activators or precursors of bioactive substances. Our earlier work has shown an increased esterification of neutral lipids of HT-29 cells during butyrate-induced differentiation (M. Madesh, O. Benard, K.A. Balasubramanian, Butyrate-induced alteration in lipid composition of human colon cell line HT-29, Biochem. Mol. Biol. Int. 38 (1996) 659-664). In this report we show that there is an increase in phospholipase D (PLD) activity during butyrate-induced differentiation of HT-29 cells as indicated by the formation of phosphatidic acid (PA). When the control and butyrate-treated cell homogenates were incubated in vitro with 1 mM Ca2+, the increase in PA formation was higher than in butyrate-treated cells. This PA was formed due to PLD activity that was confirmed by the generation of phosphatidylethanol by in vitro incubation of HT-29 cell homogenates in the presence of ethanol. The formation of PA was associated with a decrease in phosphatidylcholine (PC) and phosphatidylethanolamine (PE). This study has shown an increase in PLD activity associated with the differentiation of HT-29 cells. Copyright (C) 1998 Elsevier Science Ireland Ltd.

AB - Phospholipids are important constituents of biomembrane components and are supposed to function as enzyme activators or precursors of bioactive substances. Our earlier work has shown an increased esterification of neutral lipids of HT-29 cells during butyrate-induced differentiation (M. Madesh, O. Benard, K.A. Balasubramanian, Butyrate-induced alteration in lipid composition of human colon cell line HT-29, Biochem. Mol. Biol. Int. 38 (1996) 659-664). In this report we show that there is an increase in phospholipase D (PLD) activity during butyrate-induced differentiation of HT-29 cells as indicated by the formation of phosphatidic acid (PA). When the control and butyrate-treated cell homogenates were incubated in vitro with 1 mM Ca2+, the increase in PA formation was higher than in butyrate-treated cells. This PA was formed due to PLD activity that was confirmed by the generation of phosphatidylethanol by in vitro incubation of HT-29 cell homogenates in the presence of ethanol. The formation of PA was associated with a decrease in phosphatidylcholine (PC) and phosphatidylethanolamine (PE). This study has shown an increase in PLD activity associated with the differentiation of HT-29 cells. Copyright (C) 1998 Elsevier Science Ireland Ltd.

KW - Butyrate

KW - Differentiation

KW - HT-29 cells

KW - Phospholipase D

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U2 - 10.1016/S0304-3835(98)00172-4

DO - 10.1016/S0304-3835(98)00172-4

M3 - Article

C2 - 10397465

AN - SCOPUS:0032561285

SN - 0304-3835

VL - 132

SP - 141

EP - 146

JO - Cancer Letters

JF - Cancer Letters

IS - 1-2

ER -

Increased phospholipase D activity in butyrate-induced differentiation of HT-29 cells

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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