@inbook{83e8ef20835f425195c3d03446de18cf,
title = "Imaging the glycome in living systems",
abstract = "The glycome, the full complement of glycans that cells produce, is an attractive target for molecular imaging. Imaging of the glycome in living systems has recently been enabled via bioorthogonal chemical reporter-based approaches. In this chapter, we describe two approaches to introduce bioorthogonal chemical reporters (tags) onto cell surface fucosylated glycans and glycans bearing LacNAc disaccharides, respectively. The tagged glycans can then be conjugated to imaging probes via bioorthogonal click chemistry. Similar approaches can be extended to image other sectors of the glycome in living systems.",
keywords = "bioorthogonal, chemoenzymatic labeling, glycans, metabolic labeling",
author = "Boyangzi Li and Feiyan Mock and Peng Wu",
note = "Funding Information: Research in the authors{\textquoteright} laboratory was supported by the National Institutes of Health (GM080585 and GM093282), the Mizutani Foundation for Glycoscience, and DuPont (DuPont Young Professor Award).",
year = "2012",
doi = "10.1016/B978-0-12-388448-0.00029-2",
language = "English (US)",
series = "Methods in Enzymology",
publisher = "Academic Press Inc.",
pages = "401--419",
booktitle = "Methods in Enzymology",
}