Identification of the bromopyruvate-sensitive glutamate within the active site of 2-keto-3-deoxygluconate-6-P aldolase

H. Paul Meloche; Claire T. Monti; Ruth A. Hogue-Angeletti

doi:10.1016/0006-291X(78)90746-5

Identification of the bromopyruvate-sensitive glutamate within the active site of 2-keto-3-deoxygluconate-6-P aldolase

H. Paul Meloche, Claire T. Monti, Ruth A. Hogue-Angeletti

Developmental & Molecular Biology

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Bromopyruvate inactivates 2-keto-3-deoxygluconate-6-P aldolase by a mechanism in which the reagent is incorporated by esterification. A tryptic peptide derived from inactivated enzyme has the sequence Thr-Leu-Glu^*-Val-Thr-Leu-Arg. Derivatization of the γ-carboxyl of the single glutamate by bromopyruvate was confirmed by Lossen rearrangement in which the glutamate γ-ester was converted to 2,4-diamino butyrate.

Original language	English (US)
Pages (from-to)	589-594
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	84
Issue number	3
DOIs	https://doi.org/10.1016/0006-291X(78)90746-5
State	Published - Oct 16 1978

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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AU - Monti, Claire T.

AU - Hogue-Angeletti, Ruth A.

PY - 1978/10/16

Y1 - 1978/10/16

N2 - Bromopyruvate inactivates 2-keto-3-deoxygluconate-6-P aldolase by a mechanism in which the reagent is incorporated by esterification. A tryptic peptide derived from inactivated enzyme has the sequence Thr-Leu-Glu*-Val-Thr-Leu-Arg. Derivatization of the γ-carboxyl of the single glutamate by bromopyruvate was confirmed by Lossen rearrangement in which the glutamate γ-ester was converted to 2,4-diamino butyrate.

AB - Bromopyruvate inactivates 2-keto-3-deoxygluconate-6-P aldolase by a mechanism in which the reagent is incorporated by esterification. A tryptic peptide derived from inactivated enzyme has the sequence Thr-Leu-Glu*-Val-Thr-Leu-Arg. Derivatization of the γ-carboxyl of the single glutamate by bromopyruvate was confirmed by Lossen rearrangement in which the glutamate γ-ester was converted to 2,4-diamino butyrate.

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Identification of the bromopyruvate-sensitive glutamate within the active site of 2-keto-3-deoxygluconate-6-P aldolase

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