@inbook{d53c3350ee1349b28e5a263e1a55de5b,
title = " Identification of novel mycobacterial targets for murine CD4 + T-cells by IFNγ ELISPOT ",
abstract = " Enzyme-linked immunospot (ELISPOT) is an assay used to detect secretion of cytokines from immune cells. The resolution and sensitivity of ELISPOT allow for the detection of rare T cell specificities and small quantities of molecules produced by individual cells. In this chapter, we describe an epitope screening method that uses CD4 + T cell ELISPOT assays to identify specific novel mycobacterial antigens as potential vaccine candidates. In order to screen a large number of candidate epitopes simultaneously, pools of predicted MHC class II peptides were used to identify mycobacterial specific CD4 + T cells. Using this method, we identified novel mycobacterial antigens as vaccine candidates.",
keywords = "CD4 T cell, Cytokine, ELISPOT, Epitope, Mycobacteria",
author = "Johnson, {Alison J.} and Kennedy, {Steven C.} and Ng, {Tony W.} and Porcelli, {Steven A.}",
note = "Publisher Copyright: {\textcopyright} 2018, Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2018",
doi = "10.1007/978-1-4939-8567-8_12",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "143--150",
booktitle = "Methods in Molecular Biology",
}