TY - JOUR
T1 - Identification of a Caenorhabditis elegans histone H1 gene family. Characterization of a family member containing an intron and encoding a poly(A)+ mRNA
AU - Sanicola, Michele
AU - Ward, Samuel
AU - Childs, Geoffrey
AU - Emmons, Scott W.
N1 - Funding Information:
The authors thank Ur J. R. Vanfleterenf br supplying histonep roteinsa nd communicating results before publication. M. S. thanks and acknowledges the help of A. Silverman. a summer student. for technical assistance during the initial stages of this project. This work was supported by grant no. GM11301 from the National Institutes of Health to G.C. and S.E. and by grant no. GM25243 to S.W.G.C. is supported by an Irma T. Hirsch1 Monique Weill-Caulier Career Scientist Award. and SE. by a, Harry Winston Established Fellowship for Research of the American Heart Association.
PY - 1990/3/20
Y1 - 1990/3/20
N2 - The isolation and properties of a gene encoding a histone H1 protein of Caenorhabditis elegans, his-24, are described. The predicted protein sequence is similar to histone H1 proteins of other eukaryotes. However, the gene structure of his-24 is atypical for a histone H1 gene; it contains an intron and encodes a polyadenylated mRNA. A family of approximately five histone H1 genes is defined by cross-hybridization to his-24. All appear to encode polyadenylated mRNAs. One gene is expressed specifically in male germ cells. These histone H1 genes are dispersed individually in the genome, apart from the previously described clusters of core histone genes (H2A, H2B, H3 and H4), which probably all encode non-polyadenylated mRNAs. This histone gene organization, with clustered core histone genes, encoding non-polyadenylated transcripts, and dispersed, histone H1 genes from which it appears only polyadenylated messages arise, suggests that C. elegans is at a stage of evolution of the histone gene family intermediate between lower eukaryotes (e.g. yeast) and the most advanced forms.
AB - The isolation and properties of a gene encoding a histone H1 protein of Caenorhabditis elegans, his-24, are described. The predicted protein sequence is similar to histone H1 proteins of other eukaryotes. However, the gene structure of his-24 is atypical for a histone H1 gene; it contains an intron and encodes a polyadenylated mRNA. A family of approximately five histone H1 genes is defined by cross-hybridization to his-24. All appear to encode polyadenylated mRNAs. One gene is expressed specifically in male germ cells. These histone H1 genes are dispersed individually in the genome, apart from the previously described clusters of core histone genes (H2A, H2B, H3 and H4), which probably all encode non-polyadenylated mRNAs. This histone gene organization, with clustered core histone genes, encoding non-polyadenylated transcripts, and dispersed, histone H1 genes from which it appears only polyadenylated messages arise, suggests that C. elegans is at a stage of evolution of the histone gene family intermediate between lower eukaryotes (e.g. yeast) and the most advanced forms.
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U2 - 10.1016/0022-2836(90)90123-4
DO - 10.1016/0022-2836(90)90123-4
M3 - Article
C2 - 1969492
AN - SCOPUS:0025318295
SN - 0022-2836
VL - 212
SP - 259
EP - 268
JO - Journal of Molecular Biology
JF - Journal of Molecular Biology
IS - 2
ER -