TY - JOUR
T1 - Identification and characterization of a new source of adult human neural progenitors
AU - Behnan, Jinan
AU - Stangeland, Biljana
AU - Langella, Tiziana
AU - Finocchiaro, Gaetano
AU - Tringali, Giovanni
AU - Meling, Torstein R.
AU - Murrell, Wayne
N1 - Funding Information:
We thank the patients for donating samples. We thank the Institute for Surgical Research for support of research infrastructure; further we thank Grazyna Babinska and Yan Zhang for technical support. We thank Ana H Barragan Lid and Thea Charlotte Smedsrud at the Genomics Core Facility of the Radium Hospital. We thank Lars Eide for discussing experiments and reading the manuscript. We also thank Jan Helge Solbakk and Peter Kierulf for guidance and support. This work was funded by Helse Sør-Øst and Oslo University Hospital.
Funding Information:
Acknowledgements. We thank the patients for donating samples. We thank the Institute for Surgical Research for support of research infrastructure; further we thank Grazyna Babinska and Yan Zhang for technical support. We thank Ana H Barragan Lid and Thea Charlotte Smedsrud at the Genomics Core Facility of the Radium Hospital. We thank Lars Eide for discussing experiments and reading the manuscript. We also thank Jan Helge Solbakk and Peter Kierulf for guidance and support. This work was funded by Helse Sør-Øst and Oslo University Hospital.
Publisher Copyright:
© The Author(s) 2017.
PY - 2017
Y1 - 2017
N2 - Adult neural progenitor cells (aNPCs) are a potential source for cell based therapy for neurodegenerative diseases and traumatic brain injuries. These cells have been traditionally isolated from hippocampus, subventricular zone and white matter. However, there is still a need for an easily accessible source with better yield to counter the limitations of small surgical samples of previously characterized aNPCs. Here we show that ultrasonic aspirate (UA) samples currently considered as ‘biological waste after surgery,' offer a good source for aNPCs. Furthermore, we show that culture conditions dictated the phenotype of cells across patients. The neurosphere-enriched cells were more similar to freshly isolated brain cells, while cells expanded adherently in serum conditions were similar to mesenchymal stem cells. However, cells expanded in these adherent conditions expressed some NPC and glial markers in addition to active canonical Wnt signaling. This suggests a mesenchymal-neuroectodermal hybrid nature of these cells. Finally, we show that UA-NPCs are comparable to those from neurogenic regions. Our findings suggest that UA samples can be used as a source for fresh and in vitro propagated aNPCs that could have various clinical applications.
AB - Adult neural progenitor cells (aNPCs) are a potential source for cell based therapy for neurodegenerative diseases and traumatic brain injuries. These cells have been traditionally isolated from hippocampus, subventricular zone and white matter. However, there is still a need for an easily accessible source with better yield to counter the limitations of small surgical samples of previously characterized aNPCs. Here we show that ultrasonic aspirate (UA) samples currently considered as ‘biological waste after surgery,' offer a good source for aNPCs. Furthermore, we show that culture conditions dictated the phenotype of cells across patients. The neurosphere-enriched cells were more similar to freshly isolated brain cells, while cells expanded adherently in serum conditions were similar to mesenchymal stem cells. However, cells expanded in these adherent conditions expressed some NPC and glial markers in addition to active canonical Wnt signaling. This suggests a mesenchymal-neuroectodermal hybrid nature of these cells. Finally, we show that UA-NPCs are comparable to those from neurogenic regions. Our findings suggest that UA samples can be used as a source for fresh and in vitro propagated aNPCs that could have various clinical applications.
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U2 - 10.1038/CDDIS.2017.368
DO - 10.1038/CDDIS.2017.368
M3 - Article
C2 - 28796246
AN - SCOPUS:85046050437
SN - 2041-4889
VL - 8
JO - Cell Death and Disease
JF - Cell Death and Disease
IS - 8
M1 - e2991
ER -
