@inproceedings{53b4882042ca49daaeccf86bb0c52f17,
title = "High sensitivity MEMS biosensor for monitoring cell attachment",
abstract = "This paper presents the fabrication and testing of a novel microelectromechanical (MEMS) biosensor based on live cells. The biosensor combines two biosensing techniques; resonant frequency measurements and electric cellsubstrate impedance sensing (ECIS) on a single device. The sensor is based on the innovative placement of the working microelectrode for ECIS technique as the upper electrode of a quartz crystal microbalance (QCM) resonator. This hybrid biosensor was tested with bovine aortic endothelial cells with different seeding densities. The cell attachment and spreading was monitored with both sensors; the QCM and the ECIS technique. After the cells form a monolayer the values of the impedance and resonant frequency measurements are constant. The optimal cell seeding density with minimal time required to attach and form a monolayer was observed to be 1.5×104 cells/cm2. This biosensor monitors the cells attachment and viability and could be used for screening toxicants in drinking water.",
author = "Fei Liu and Fang Li and Spray, \{David C.\} and Nordin, \{Anis Nurashikin\} and Ioana Voiculescu",
year = "2012",
doi = "10.1115/IMECE2012-85613",
language = "English (US)",
isbn = "9780791845257",
series = "ASME International Mechanical Engineering Congress and Exposition, Proceedings (IMECE)",
publisher = "American Society of Mechanical Engineers (ASME)",
number = "PARTS A AND B",
pages = "67--71",
booktitle = "Micro- and Nano-Systems Engineering and Packaging",
edition = "PARTS A AND B",
note = "ASME 2012 International Mechanical Engineering Congress and Exposition, IMECE 2012 ; Conference date: 09-11-2012 Through 15-11-2012",
}