Hematopoietic Stem and Progenitor Cell Identification and Transplantation in Zebrafish

Ellen Fraint, Peng Lv, Feng Liu, Teresa V. Bowman, Owen J. Tamplin

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations


The zebrafish as a model organism is well known for its versatile genetics, rapid development, and straightforward live imaging. It is an excellent model to study hematopoiesis because of its highly conserved ontogeny and gene regulatory networks. Recently developed highly specific transgenic reporter lines have allowed direct imaging and tracking of hematopoietic stem and progenitor cells (HSPCs) in live zebrafish. These reporter lines can also be used for fluorescence-activated cell sorting (FACS) of HSPCs. Similar to mammalian models, HSPCs can be transplanted to reconstitute the entire hematopoietic system of zebrafish recipients. However, the zebrafish provides unique advantages to study HSPC biology, such as transplants into embryos and high-throughput chemical screening. This chapter will outline the methods needed to identify, isolate, and transplant HSPCs in zebrafish.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages17
StatePublished - 2023

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • HSPC transplantation
  • Hematopoietic stem and progenitor cells (HSPCs)
  • Live imaging
  • Transgenic reporter lines
  • Zebrafish

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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