@article{df8f32f38cc54f1b989fad764342acb5,
title = "Gut microbiota and plasma metabolites associated with diabetes in women with, or at high risk for, HIV infection",
abstract = " Background: Gut microbiota alteration has been implicated in HIV infection and metabolic disorders. The relationship between gut microbiota and diabetes has rarely been studied in HIV-infected individuals, who have excess risk of metabolic disorders. Methods: Our study during 2015–2016 enrolled predominantly African Americans and Hispanics in the Women's Interagency HIV Study. We studied 28 women with long-standing HIV infection under antiretroviral therapy and 20 HIV-uninfected, but at high risk of infection, women (16 HIV+ and 6 HIV- with diabetes). Fecal samples were analyzed by sequencing prokaryotic16S rRNA gene. Plasma metabolomics profiling was performed by liquid chromatography-tandem mass spectrometry. Findings: No significant differences in bacterial α- or β-diversity were observed by diabetes or HIV serostatus (all P >.1). Relative abundances of four genera (Finegoldia, Anaerococcus, Sneathia, and Adlercreutzia) were lower in women with diabetes compared to those without diabetes (all P <.01). In women with diabetes, plasma levels of several metabolites in tryptophan catabolism (e,g., kynurenine/tryptophan ratio), branched-chain amino acid and proline metabolism pathways were higher, while glycerophospholipids were lower (all P <.05). Results were generally consistent between HIV-infected and HIV-uninfected women, and no significant modification effects by HIV serostatus were observed (all P interaction > 0.05). Anaerococcus, known to produce butyrate which is involved in anti-inflammation and glucose metabolism, showed an inverse correlation with kynurenine/tryptophan ratio (r = −0.38, P <.01). Interpretation: Among women with or at high risk for HIV infection, diabetes is associated with gut microbiota and plasma metabolite alteration, including depletion of butyrate-producing bacterial population along with higher tryptophan catabolism. Fund: NHLBI (K01HL129892, R01HL140976) and FMF.",
keywords = "Diabetes, Gut microbiota, HIV, Metabolite",
author = "Moon, {Jee Young} and Zolnik, {Christine P.} and Zheng Wang and Yunping Qiu and Mykhaylo Usyk and Tao Wang and Kizer, {Jorge R.} and Landay, {Alan L.} and Kurland, {Irwin J.} and Kathryn Anastos and Kaplan, {Robert C.} and Burk, {Robert D.} and Qibin Qi",
note = "Funding Information: This study was supported by the National Heart, Lung, and Blood Institute (NHLBI) K01HL129892 and R01HL140976 , and Feldstein Medical Foundation Research Grant to Q.Q. R.C·K was supported by NHLBI 5R01HL126543 , R01 HL132794 and the National Institute on Mental Health (NIMH) 5R01MD011389-03 , and J.R.K. was supported by NHLBI R01 HL132794 and K24 HL135413. Other funding sources include NHLBI R01HL083760, and R01HL095140, the National Institute of Allergy and Infectious Diseases (NIAID) U01 AI035004 , and the Einstein Cancer Research Center ( P30CA013330 ), the Einstein Liver Research Center ( P30DK041296 ), the Einstein-Rockefeller-CUNY Center for AIDS Research funded by the NIAID ( P30AI124414 ), and the Stable Isotope and Metabolomics Core Facility of the Einstein-Mount Sinai Diabetes Research Center (ES-DRC) of the Albert Einstein College of Medicine funded by National Cancer Institute (P60DK020541). Funding Information: Data in this manuscript were collected by the Women's Interagency HIV Study (WIHS). The contents of this publication are solely the responsibility of the authors and do not represent the official views of the National Institutes of Health (NIH). WIHS (Principal Investigators): UAB-MS WIHS (Mirjam-Colette Kempf and Deborah Konkle-Parker), U01-AI-103401; Atlanta WIHS (Ighovwerha Ofotokun and Gina Wingood), U01-AI-103408; Bronx WIHS (Kathryn Anastos and Anjali Sharma), U01-AI-035004; Brooklyn WIHS (Howard Minkoff and Deborah Gustafson), U01-AI-031834; Chicago WIHS (Mardge Cohen and Audrey French), U01-AI-034993; Metropolitan Washington WIHS (Seble Kassaye), U01-AI-034994; Miami WIHS (Margaret Fischl and Lisa Metsch), U01-AI-103397; UNC WIHS (Adaora Adimora), U01-AI-103390; Connie Wofsy Women's HIV Study, Northern California (Ruth Greenblatt, Bradley Aouizerat, and Phyllis Tien), U01-AI-034989; WIHS Data Management and Analysis Center (Stephen Gange and Elizabeth Golub), U01-AI-042590; Southern California WIHS (Joel Milam), U01-HD-032632 (WIHS I – WIHS IV). The WIHS is funded primarily by the National Institute of Allergy and Infectious Diseases (NIAID), with additional co-funding from the Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD), the National Cancer Institute (NCI), the National Institute on Drug Abuse (NIDA), and the National Institute on Mental Health (NIMH). Targeted supplemental funding for specific projects is also provided by the National Institute of Dental and Craniofacial Research (NIDCR), the National Institute on Alcohol Abuse and Alcoholism (NIAAA), the National Institute on Deafness and other Communication Disorders (NIDCD), and the NIH Office of Research on Women's Health. WIHS data collection is also supported by UL1-TR000004 (UCSF CTSA), UL1-TR000454 (Atlanta CTSA), and P30-AI-050410 (UNC CFAR). Publisher Copyright: {\textcopyright} 2018 The Authors",
year = "2018",
month = nov,
doi = "10.1016/j.ebiom.2018.10.037",
language = "English (US)",
volume = "37",
pages = "392--400",
journal = "EBioMedicine",
issn = "2352-3964",
publisher = "Elsevier BV",
}
