TY - JOUR
T1 - Growth of murine sarcoma virus-transformed rat kidney cells in nude mice
T2 - Absence of induction of host endogenous viruses
AU - Bilello, John A.
AU - Freedman, Victoria H.
AU - Shin, Seung Il
N1 - Funding Information:
ABBREVIATIONS USED: SV40 = simian virus 40; Ki-MuSV = Kirsten murine sarcoma virus(es) (MuSV); NRK = normal rat kidney; (M-MuSV)NRK = Moloney murine sarcoma virus (M-MuSV)-transformed NRK cell line; WoLV = woolly monkey leukemia virus; RT = reverse transcriptase. Received July 26, 1976; accepted November 9, 1976. 2 Supported by Public Health Service (PHS) grants GM21014 and GM19100 from the National Institute of General Medical Sciences. 3 Department of Molecular Biology, Albert Einstein College of Medicine, Bronx, N.Y. 10461. 4 Supported by PHS training grant 5 TOI GM02204 awarded to the Division of Biological Sciences, Albert Einstein College of Medicine, from the National Institute of General Medical Sciences. 5 Department of Genetics, Albert Einstein College of Medicine, 1300 Morris Park Ave., Bronx, N.Y. 10461. 6 Supported by PHS training grant 5 T32 CA09l73 in Immunology and Immunooncology from the National Cancer Institute. 7 Recipient of a Faculty Research Award from the American Cancer Society, Inc. 8 Address reprint requests to Dr. S. Shin. 9 We acknowledge the expert technical assistance of Andre Brown.
PY - 1977/6
Y1 - 1977/6
N2 - Clonal isolates of the normal rat kidney cell line (NRK) transformed by a defective murine sarcoma virus (Kirsten strain) were injected into nude mice of BALB/c background to determine whether the growth of these cells as tumors was accompanied by the induction of host endogenous type C viruses. All the virus-transformed clones produced rapidly growing tumors in nude mice, but neither the induction of mouse endogenous viruses nor the rescue and spread of the transforming sarcoma virus were observed during the growth of tumors. The degree of expression of the tumor virus structural proteins in the transformed cells did not determine the cellular phenotype with regard to tumorigenicity in nude mice, nor did it modify the cellular growth properties in vitro. Consistent with earlier observations with simian virus 40-transformed mouse and rat cells, the ability of sarcoma virus-transformed NRK cells to initiate tumor growth in nude mice appeared to be correlated with anchorage-independent growth in vitro.
AB - Clonal isolates of the normal rat kidney cell line (NRK) transformed by a defective murine sarcoma virus (Kirsten strain) were injected into nude mice of BALB/c background to determine whether the growth of these cells as tumors was accompanied by the induction of host endogenous type C viruses. All the virus-transformed clones produced rapidly growing tumors in nude mice, but neither the induction of mouse endogenous viruses nor the rescue and spread of the transforming sarcoma virus were observed during the growth of tumors. The degree of expression of the tumor virus structural proteins in the transformed cells did not determine the cellular phenotype with regard to tumorigenicity in nude mice, nor did it modify the cellular growth properties in vitro. Consistent with earlier observations with simian virus 40-transformed mouse and rat cells, the ability of sarcoma virus-transformed NRK cells to initiate tumor growth in nude mice appeared to be correlated with anchorage-independent growth in vitro.
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U2 - 10.1093/jnci/58.6.1691
DO - 10.1093/jnci/58.6.1691
M3 - Article
C2 - 194043
AN - SCOPUS:0017640040
SN - 0027-8874
VL - 58
SP - 1691
EP - 1694
JO - Journal of the National Cancer Institute
JF - Journal of the National Cancer Institute
IS - 6
ER -
