Generation of SMURF2 knockout human cells using the CRISPR/Cas9 system

Dhanoop Manikoth Ayyathan; Nataša Ilić; Hava Gil-Henn; Michael Blank

doi:10.1016/j.ab.2017.05.024

Generation of SMURF2 knockout human cells using the CRISPR/Cas9 system

Dhanoop Manikoth Ayyathan
, Nataša Ilić
, Hava Gil-Henn
, Michael Blank

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

The HECT domain E3 ubiquitin ligase SMURF2 regulates stability of several key protein targets involved in tumorigenesis, cell proliferation, migration, differentiation, and senescence. While altered levels and aberrant cellular distribution of SMURF2 were reported in different types of cancer, its role in tumorigenesis is far from understood. To elucidate the role of SMURF2 in cancer, appropriate human cancer cell models are needed. Here, we describe approaches that can be used to generate human normal and cancer cell strains knocked-out for SMURF2 using the clustered regularly interspaced short palindromic repeats (CRISPR/Cas9) gene-editing technology.

Original language	English (US)
Pages (from-to)	56-59
Number of pages	4
Journal	Analytical Biochemistry
Volume	531
DOIs	https://doi.org/10.1016/j.ab.2017.05.024
State	Published - Aug 15 2017
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

CRISPR/Cas9
Human cells
Knockout
SMURF2

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1016/j.ab.2017.05.024

Cite this

@article{e13e7a943f31466d968250d44959fafa,

title = "Generation of SMURF2 knockout human cells using the CRISPR/Cas9 system",

abstract = "The HECT domain E3 ubiquitin ligase SMURF2 regulates stability of several key protein targets involved in tumorigenesis, cell proliferation, migration, differentiation, and senescence. While altered levels and aberrant cellular distribution of SMURF2 were reported in different types of cancer, its role in tumorigenesis is far from understood. To elucidate the role of SMURF2 in cancer, appropriate human cancer cell models are needed. Here, we describe approaches that can be used to generate human normal and cancer cell strains knocked-out for SMURF2 using the clustered regularly interspaced short palindromic repeats (CRISPR/Cas9) gene-editing technology.",

keywords = "CRISPR/Cas9, Human cells, Knockout, SMURF2",

author = "\{Manikoth Ayyathan\}, Dhanoop and Nata{\v s}a Ili{\'c} and Hava Gil-Henn and Michael Blank",

note = "Publisher Copyright: {\textcopyright} 2017 Elsevier Inc.",

year = "2017",

month = aug,

day = "15",

doi = "10.1016/j.ab.2017.05.024",

language = "English (US)",

volume = "531",

pages = "56--59",

journal = "Analytical Biochemistry",

issn = "0003-2697",

publisher = "Academic Press Inc.",

}

TY - JOUR

T1 - Generation of SMURF2 knockout human cells using the CRISPR/Cas9 system

AU - Manikoth Ayyathan, Dhanoop

AU - Ilić, Nataša

AU - Gil-Henn, Hava

AU - Blank, Michael

PY - 2017/8/15

Y1 - 2017/8/15

N2 - The HECT domain E3 ubiquitin ligase SMURF2 regulates stability of several key protein targets involved in tumorigenesis, cell proliferation, migration, differentiation, and senescence. While altered levels and aberrant cellular distribution of SMURF2 were reported in different types of cancer, its role in tumorigenesis is far from understood. To elucidate the role of SMURF2 in cancer, appropriate human cancer cell models are needed. Here, we describe approaches that can be used to generate human normal and cancer cell strains knocked-out for SMURF2 using the clustered regularly interspaced short palindromic repeats (CRISPR/Cas9) gene-editing technology.

AB - The HECT domain E3 ubiquitin ligase SMURF2 regulates stability of several key protein targets involved in tumorigenesis, cell proliferation, migration, differentiation, and senescence. While altered levels and aberrant cellular distribution of SMURF2 were reported in different types of cancer, its role in tumorigenesis is far from understood. To elucidate the role of SMURF2 in cancer, appropriate human cancer cell models are needed. Here, we describe approaches that can be used to generate human normal and cancer cell strains knocked-out for SMURF2 using the clustered regularly interspaced short palindromic repeats (CRISPR/Cas9) gene-editing technology.

KW - CRISPR/Cas9

KW - Human cells

KW - Knockout

KW - SMURF2

UR - https://www.scopus.com/pages/publications/85019695300

UR - https://www.scopus.com/pages/publications/85019695300#tab=citedBy

U2 - 10.1016/j.ab.2017.05.024

DO - 10.1016/j.ab.2017.05.024

M3 - Article

C2 - 28552759

AN - SCOPUS:85019695300

SN - 0003-2697

VL - 531

SP - 56

EP - 59

JO - Analytical Biochemistry

JF - Analytical Biochemistry

ER -

Generation of SMURF2 knockout human cells using the CRISPR/Cas9 system

Abstract

UN SDGs

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this