Generating hypoimmunogenic human embryonic stem cells by the disruption of beta 2-microglobulin

Pengfei Lu; Jijun Chen; Lixiazi He; Jiangtao Ren; Haide Chen; Lingjun Rao; Qinggang Zhuang; Hui Li; Lei Li; Lei Bao; Ji He; Wei Zhang; Faming Zhu; Chun Cui; Lei Xiao

doi:10.1007/s12015-013-9457-0

Generating hypoimmunogenic human embryonic stem cells by the disruption of beta 2-microglobulin

Pengfei Lu
, Jijun Chen
, Lixiazi He
, Jiangtao Ren
, Haide Chen
, Lingjun Rao
, Qinggang Zhuang
, Hui Li
, Lei Li
, Lei Bao
, Ji He
, Wei Zhang
, Faming Zhu
, Chun Cui
, Lei Xiao

Research output: Contribution to journal › Article › peer-review

67 Scopus citations

Abstract

Immune rejection hinders the application of human embryonic stem cells (hESCs) in transplantation therapy. Human leukocyte antigens (HLAs) on the cell surface are the major cause of graft rejection. In this study, we generatedHLA class I-deficient hESCs via disruption of beta 2-microglobulin (β2m), the light chain of HLA Class I. We found that HLA class I proteins were not present on the cell surface of β2mnull hESCs. These cells showed the same pluripotency as wildtype hESCs and demonstrated hypoimmunogenicity. Thus, HLA class I-deficient hESCs might serve as an unlimited cell source for the generation of universally compatible “off-the-shelf” cell grafts, tissues or organs in the future.

Original language	English (US)
Article number	A007
Pages (from-to)	806-813
Number of pages	8
Journal	Stem Cell Reviews and Reports
Volume	9
Issue number	6
DOIs	https://doi.org/10.1007/s12015-013-9457-0
State	Published - Dec 2015
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Beta 2-microglobulin
Human embryonic stem cells
Human leukocyte antigens
Hypoimmunogenicity
IPS cells
Immune rejection

ASJC Scopus subject areas

Cell Biology
Cancer Research

Access to Document

10.1007/s12015-013-9457-0

Cite this

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title = "Generating hypoimmunogenic human embryonic stem cells by the disruption of beta 2-microglobulin",

abstract = "Immune rejection hinders the application of human embryonic stem cells (hESCs) in transplantation therapy. Human leukocyte antigens (HLAs) on the cell surface are the major cause of graft rejection. In this study, we generatedHLA class I-deficient hESCs via disruption of beta 2-microglobulin (β2m), the light chain of HLA Class I. We found that HLA class I proteins were not present on the cell surface of β2mnull hESCs. These cells showed the same pluripotency as wildtype hESCs and demonstrated hypoimmunogenicity. Thus, HLA class I-deficient hESCs might serve as an unlimited cell source for the generation of universally compatible “off-the-shelf” cell grafts, tissues or organs in the future.",

keywords = "Beta 2-microglobulin, Human embryonic stem cells, Human leukocyte antigens, Hypoimmunogenicity, IPS cells, Immune rejection",

author = "Pengfei Lu and Jijun Chen and Lixiazi He and Jiangtao Ren and Haide Chen and Lingjun Rao and Qinggang Zhuang and Hui Li and Lei Li and Lei Bao and Ji He and Wei Zhang and Faming Zhu and Chun Cui and Lei Xiao",

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year = "2015",

month = dec,

doi = "10.1007/s12015-013-9457-0",

language = "English (US)",

volume = "9",

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TY - JOUR

T1 - Generating hypoimmunogenic human embryonic stem cells by the disruption of beta 2-microglobulin

AU - Lu, Pengfei

AU - Chen, Jijun

AU - He, Lixiazi

AU - Ren, Jiangtao

AU - Chen, Haide

AU - Rao, Lingjun

AU - Zhuang, Qinggang

AU - Li, Hui

AU - Li, Lei

AU - Bao, Lei

AU - He, Ji

AU - Zhang, Wei

AU - Zhu, Faming

AU - Cui, Chun

AU - Xiao, Lei

N1 - Publisher Copyright: © Springer Science+Business Media New York 2013

PY - 2015/12

Y1 - 2015/12

N2 - Immune rejection hinders the application of human embryonic stem cells (hESCs) in transplantation therapy. Human leukocyte antigens (HLAs) on the cell surface are the major cause of graft rejection. In this study, we generatedHLA class I-deficient hESCs via disruption of beta 2-microglobulin (β2m), the light chain of HLA Class I. We found that HLA class I proteins were not present on the cell surface of β2mnull hESCs. These cells showed the same pluripotency as wildtype hESCs and demonstrated hypoimmunogenicity. Thus, HLA class I-deficient hESCs might serve as an unlimited cell source for the generation of universally compatible “off-the-shelf” cell grafts, tissues or organs in the future.

AB - Immune rejection hinders the application of human embryonic stem cells (hESCs) in transplantation therapy. Human leukocyte antigens (HLAs) on the cell surface are the major cause of graft rejection. In this study, we generatedHLA class I-deficient hESCs via disruption of beta 2-microglobulin (β2m), the light chain of HLA Class I. We found that HLA class I proteins were not present on the cell surface of β2mnull hESCs. These cells showed the same pluripotency as wildtype hESCs and demonstrated hypoimmunogenicity. Thus, HLA class I-deficient hESCs might serve as an unlimited cell source for the generation of universally compatible “off-the-shelf” cell grafts, tissues or organs in the future.

KW - Beta 2-microglobulin

KW - Human embryonic stem cells

KW - Human leukocyte antigens

KW - Hypoimmunogenicity

KW - IPS cells

KW - Immune rejection

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UR - https://www.scopus.com/pages/publications/84937518844#tab=citedBy

U2 - 10.1007/s12015-013-9457-0

DO - 10.1007/s12015-013-9457-0

M3 - Article

C2 - 23934228

AN - SCOPUS:84937518844

SN - 2629-3269

VL - 9

SP - 806

EP - 813

JO - Stem Cell Reviews and Reports

JF - Stem Cell Reviews and Reports

IS - 6

M1 - A007

ER -

Generating hypoimmunogenic human embryonic stem cells by the disruption of beta 2-microglobulin

Abstract

UN SDGs

Keywords

ASJC Scopus subject areas

Access to Document

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