Gene expression within a dynamic nuclear landscape

Yaron Shav-Tal, Xavier Darzacq, Robert H. Singer

Research output: Contribution to journalReview articlepeer-review

29 Scopus citations


Molecular imaging in living cells or organisms now allows us to observe macromolecular assemblies with a time resolution sufficient to address cause-and-effect relationships on specific molecules. These emerging technologies have gained much interest from the scientific community since they have been able to reveal novel concepts in cell biology, thereby changing our vision of the cell. One main paradigm is that cells stochastically vary, thus implying that population analysis may be misleading. In fact, cells should be analyzed within time-resolved single-cell experiments rather than being compared to other cells within a population. Technological imaging developments as well as the stochastic events present in gene expression have been reviewed. Here, we discuss how the structural organization of the nucleus is revealed using noninvasive single-cell approaches, which ultimately lead to the resolution required for the analysis of highly controlled molecular processes taking place within live cells. We also describe the efforts being made towards physiological approaches within the context of living organisms.

Original languageEnglish (US)
Pages (from-to)3469-3479
Number of pages11
JournalEMBO Journal
Issue number15
StatePublished - Aug 9 2006


  • Chromatin
  • Nuclear bodies
  • Nuclear dynamics
  • Nucleolus
  • Transcription
  • mRNA

ASJC Scopus subject areas

  • General Neuroscience
  • Molecular Biology
  • General Biochemistry, Genetics and Molecular Biology
  • General Immunology and Microbiology


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